Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study according to EU/OECd-guidelines.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
14.7 °dH (= 262.4 mg/L CaCO3)
Test temperature:
20.6 - 21.0
pH:
7.8
Nominal and measured concentrations:
0.13, 0.28, 0.62, 1.36 and 3.00 mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
0.541 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: not determined due to mathematical reasons or inappropriate data
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.522 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 0.667-0.863
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
2.451 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: not determined due to mathematical reasons or inappropriate data
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
2.415 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 0.667-0.863
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
1.21 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: not determined due to mathematical reasons or inappropriate data
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.757 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 0.667-0.863

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
An analysis of the immobilisation rates (by probit analysis) gave the following results:
Time [h] EC 0 [mg/L] EC 100 [mg/L] EC 50 [mg/L] 95 % confidence limits [mg/L]
24 0.541 2.451 1.210 n.d.
48 0.522 2.415 0.757 0.667-0.863
n.d.: not determined due to mathematical reasons or inappropriate data