Magnesium bis(dinonylnaphthalenesulphonate)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

February 23 - April 4, 1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was not conducted to meet GLP regulations, however, the test followed standard guideline procedures (US Federal Hazardous Substances Labeling Act (FHSLA) 16 CFR 1500).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Acute Dermal Toxicity: FHSLA 16 CFR 1500.

Deviations:

not specified

GLP compliance:

no

Test type:

other: Limit test

Test material

Test material information

Constituent 1

Reference substance name:

Barium bis(di C8-C10, branched, C9 rich, alkylnaphthalenesulphonate)

IUPAC Name:

Barium bis(di C8-C10, branched, C9 rich, alkylnaphthalenesulphonate)

Test material form:

other: as solution in light petroleum distillates

Details on test material:

A viscous brown liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

The animals were housed 1 per cage. Feed and water were provided ad-libitum after dosing.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

The rabbits were prepared by clipping the skin of the trunk free of hair. Epidermal abrasions were made over a 5-6 cm2 area on 3 males and 2 females. The remaining five rabbits were not abraded. A non-permeable patch containing 2.0 ml/kg body weight of the test material was placed over a 4-5 cm2 area on all rabbits. The patches were secured in place with tape and an elastic sleeve. The rabbits were immobilized in head stocks for 24 hours at which time the patches were removed and the rabbits returned to their cages. Feed and water were provided ad-libitum. The rabbits were
observed for 14 days.

Duration of exposure:

24 hours

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 10 mg/kg bw

Based on:

act. ingr.

Mortality:

Skin Sex Body Weight Dose Mortality
Treatment kg grams Test Day

Abraded M 2.73 55 14
Abraded M 2.60 52
Unabraded M 2.37 47 2
Unabraded M 2.72 54
Unabraded M 2.62 52 4
Abraded F 2.71 54
Abraded F 2.97 59 5
Abraded F 3.0 60
Unabraded F 2.5 50 6
Unabraded F 2.41 48

Clinical signs:

Surviving rabbits exhibited anorexia, weight
loss, debilitation, spasticity.

Body weight:

See table.

Gross pathology:

Nos. 1M, 2M, 5M - I iver necroses, enlarged kidneys
Nos. 7F, 9F - 1 iver necroses, concentrated urine,
enlarged kidneys.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Remarks:

Migrated information

Conclusions:

The Dermal LD50 of the test material is ~ 20 ml/kg for a 50% formulation. The LD50 as active ingredient is greater than 10,000 mg/kg.

Executive summary:

A dermal acute toxicity test was conducted in New Zealand Albino Rabbits. Ten rabbits (5 male / 5 female) received a dose of 20 ml/kg bw of the test substance for 24 hours and were observed for 14 days for signs of toxicity and mortality. The skin of 5 rabbits was abraded prior to dosing (5-6 cm2 area on 3 males and 2 females). The Dermal LD50 of the test material is ~ 20 ml/kg. The LD50 as active ingredient is greater than 10,000 mg/kg.