Registration Dossier

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
(Q)SAR
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction:US EPA accepted QSAR method for chemicals properties assessment.
Qualifier:
according to guideline
Guideline:
other: QSAR
Principles of method if other than guideline:
ECOSAR Program (v1.00)
GLP compliance:
no
Remarks:
not applicable to QSAR models
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: earthworm
Animal group:
other: QSAR model
Study type:
other: QSAR
Substrate type:
other: QSAR
Limit test:
no
Total exposure duration:
14 d
Test temperature:
not applicable. QSAR model
pH:
not applicable. QSAR model
Moisture:
not applicable. QSAR model
Details on test conditions:
SMILES : O(C(=NCC)S)C(C)C
CHEM : Carbamothioic acid, ethyl-, O-(1-methylethyl) ester
CAS Number: 141-98-0
MOL FOR: C6 H13 N1 O1 S1
MOL WT : 147.24
Log Kow: 3.32 (KowWin estimate)
Wat Sol: 114.4 mg/L (WskowWin estimate)


Reference substance (positive control):
no
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
186.87 mg/kg soil ww
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified
Remarks on result:
other: 1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.
Details on results:
LC50 (14-day, earthworm)= 186.870 mg/L (ppm)
1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.
LC50 (14-day, earthworm)=186.870 mg/kg soil dw
Reported statistics and error estimates:
US EPA accepted QSAR method for chemicals properties assessment.

Overview of QSARs estimations on toxicity of O-isopropyl ethylthiocarbamate (IPETC ) to soil macroorganisms

Method

 

Results

Remarks

Reference

QSAR

estimate

LC50 (14-day, earthworm)= 186.870 mg/L (ppm)

 

 

Reliable withrestrictions,

QSAR estimated data

ECOSAR Program (v1.00)

 

 

LC50 (14-day, earthworm)= 186.870 mg/kg soil dw

Validity criteria fulfilled:
yes
Remarks:
US EPA accepted QSAR method for chemicals properties assessment.
Conclusions:
LC50 (14-day, earthworm)= 186.870 mg/L (ppm)
1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.
LC50 (14-day, earthworm)=186.870 mg/kg soil dw
Executive summary:

The ECOSAR QSAR model predicts that 14-day, LC50 (14-day, earthworm) would to be 186.870 mg/L (ppm).

1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.

LC50 (14-day, earthworm)= 186.870 mg/kg soil dw

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Propan-2-ol (Isopropyl alcohol) is both reagents used in the manufacture of Reaction mass of O-isopropyl ethylthiocarbamate and n-butanol and propan-2-ol. Therefore, Propan-2-ol (Isopropyl alcohol) need to be considered in the assessment of Reaction mass of O-isopropyl ethylthiocarbamate and n-butanol and propan-2-ol .
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
not specified
GLP compliance:
not specified
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Caenorhabditis elegans
Animal group:
nematods
Details on test organisms:
SPECIES #
(NAME): 4550 Caenorhabditis elegans
(Nematode)
AGE: NR NR
LIFE STAGE: larva(e)
COMMENT: NR
Study type:
laboratory study
Substrate type:
not specified
Limit test:
no
Total exposure duration:
24 h
Test temperature:
not reported
pH:
not reported
Moisture:
not reported
Details on test conditions:
6.55 % v/v percent volume per volume
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
6.55 other: % v/v
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: % v/v - percent volume per volume
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
6.7 other: % v/v
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: % v/v - percent volume per volume
Details on results:
A 24 h LC50 of 6.5 to 6.6 % v/v - percent volume per volume has been determined for mortality to Caenorhabditis elegans of the test substance.

The toxicities of 4 short-chain alcohols--namely methanol, ethanol, iso-propanol and iso-butanol--were compared in the nematode Caenorhabditis elegans using several different ecotoxicological endpoints. Range-finding tests were conducted using transgenic PC161 worms carrying a double reporter construct (GFP plus lacZ) linked to the stress-inducible hsp16-1 promoter. These tests showed little response from the GFP reporter, but gave good dose-response curves for the lacZ reporter--showing clear induction at 0.5% v/v ethanol in an overnight assay, but only at 4% in a shorter 6-h assay.

Comparison of the short-term dose-response curves shows a confusing pattern of differences between the four alcohols tested, although dose-dependence is evident across at least part of the concentration range. Feeding inhibition assays are somewhat inconclusive with regard to alcohol type, although iso-butanol and iso-propanol appear more toxic than ethanol, while methanol is least toxic. To resolve some of the remaining ambiguities, we also used a fecundity assay to show that iso-propanol is more toxic than ethanol, and a lethality assay to show that iso-butanol is more toxic than iso-propanol.

Most of the endpoints studied are consistent with the following order of toxicity: iso-butanol > iso-propanol > ethanol > or = methanol.

Validity criteria fulfilled:
not specified
Conclusions:
A 24 h LC50 of 6.5 to 6.7 % v/v - percent volume per volume has been determined for mortality to Caenorhabditis elegans of the test substance.
Propan-2-ol (Isopropyl alcohol) is both reagents used in the manufacture of Reaction mass of O-isopropyl ethylthiocarbamate and n-butanol and propan-2-ol. Therefore, Propan-2-ol (Isopropyl alcohol) need to be considered in the assessment of Reaction mass of O-isopropyl ethylthiocarbamate and n-butanol and propan-2-ol .

Description of key information

The ECOSAR QSAR model predicts that 14-day, LC50 (14-day, earthworm) would to be 186.870 mg/L (ppm).
1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.
LC50 (14-day, earthworm)= 186.870 mg/kg soil dw

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
186.87 mg/kg soil dw
Long-term EC10, LC10 or NOEC for soil macroorganisms:
37.374 mg/kg soil dw

Additional information

The ECOSAR QSAR model predicts that 14-day, LC50 (14-day, earthworm) would to be 186.870 mg/L (ppm).

1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.

LC50 (14-day, earthworm)= 186.870 mg/kg soil dw.