Trisodium 5-[[4-chloro-6-(ethylphenylamino)-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate

Endpoint summary

Description of key information

Short-term toxicity tests are available for all the three tropic levels (fish, invertebrates and aquatic plants). In a 48 h acute fish toxicity test, FAT 40034 was observed to have 48 h LD₅₀ to trout fishes of >1000 mg/L. Similarly, in a study conducted according to OECD Guideline 202, the 48 h EC₅₀ value of the test substance FAT 40034 to Daphnia magna was determined to be >100 mg/L. The NOEC value for 48 h was determined to be 100 mg/L. Due to intrinsic properties of the substance, the test to determine the acute toxicity to aquatic algae and cyanobacteria cannot be performed. The dark coloration of the test medium and reduced light intensity in the medium modifying the conditions of test. Hence, to determine the toxicity to the aquatic plant Lemna minor, a semi-static growth inhibition test was performed. In a GLP-compliant OECD Guideline 221 study, Lemna gibba (Duckweed) exposed to FAT 40034 recorded: 7 day ErC₅₀ based upon the frond number is >100 mg/L. 7 day EyC₅₀based upon the frond numbers is >100 mg/L. 7 day ErC₅₀ based upon the frond dry weight is >100 mg/L. 7 day EyC₅₀ based upon the frond dry weight is >100 mg/L.The NOEC was 0.64 mg/L and the LOEC was >100 mg /L.Therefore, it can be concluded that there were no toxic effects at saturation. A toxicity study of FAT 40034 to activated sludge was determined in a 3-h respiration inhibition test.The IC50 value of the test substance FAT 40034 is greater than 300 mg/L. Hence, based on the available data FAT 40034 can be considered as not toxic to aquatic species.