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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study, validity criteria fulfilled;

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Isobutyl-tert.Butylether
IUPAC Name:
Isobutyl-tert.Butylether
Details on test material:
- Test substance: Isobutyl-tert.Butylether
- purity: 86.4%

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Preparation or the inoculum : The inoculum' was washed, brought to a concentration of 5 g/I dry substance and aerated during the night . 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test.

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
30 min

Test conditions

Details on test conditions:
- Incubation time : 30 min
- Test temperature : 20 ± 2 °C
- Test vessels : Erlenmeyer-vessel (nominal volume 250 ml)
- Test volume : 250 m l
- Synthetic medium : 8 ml/vessel 100-fold concentrated OECD medium

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: IUCLID4 note: "m" (measured/nominal)
Duration:
30 min
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: IUCLID4 note: "m" (measured/nominal)
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: IUCLID4 note: "m" (measured/nominal)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes