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Toxicity to aquatic algae and cyanobacteria

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Description of key information

In a study performed according to OECD TG 201 exposure of Pseudokirchneriella subcapitata to the test item gave a ErL50 (0 -72h) > 100 mg/L loading rate WAF. The NOErLR was 50 mg/L loading rate WAF. The EyL50 (0 -72h) was 67 mg/L loading rate WAF and the NOEyLR was 50 mg/L loading rate WAF.

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GUIDELINE

A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed was designed to be compatible with OECD TG 201, referenced as Method C.3 of Commission Regulation (EC) 761/2009.

METHODS

Due to the low aqueous solubility and complex nature of the test item for the purposes of the test the test item was prepared as a Water Accommodated Fraction (WAF). Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed to WAFs of the test item over a range of nominal loading rates of 6.25, 12.5, 25, 50 and 100 mg/L (three replicate flasks per concentration) for 72 hours under constant illumination and shaking at a temperature of 24 +/-1 deg C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group using a Coulter Multisizer Particle Counter.

RESULTS

Analysis of the test preparations at 0 and 72 hours showed measured test concentrations to range from 0.044 to 94 mg/L.

Given that the toxicity cannot be attributed to a single component or mixture of components but to the test item as a whole, the results were based on nominal loading rates only.

CONCLUSION

The ErL50 (0 -72h) was > 100 mg/L loading rate WAF and the NOErLR was 50 mg/L loading rate WAF. The EyL50 (0 -72h) was 67 mg/L loading rate WAF and the NOEyLR was 50 mg/L loading rate WAF.