Reaction mass of sodium 7-hydroxy-8-((E)-(4-(1-(4-((E)-(4-hydroxyphenyl)diazenyl)phenyl)cyclohexyl)phenyl)diazenyl)naphthalene-1,3-disulfonate and sodium 8,8'-(1E,1'E)-(4,4'-(cyclohexane-1,1-diyl)bis(4,1-phenylene))bis(diazene-2,1-diyl)bis(7-hydroxynaphthalene-1,3-disulfonate)

Endpoint summary

Description of key information

Substance is classified as Aquatic Chronic 3 (H412) according to the CLP Regulation (EC 1272/2008).

Short term toxicity to aquatic invertebrates: EC50 (48h): 48.9 mg/l

Toxicity to aquatic plants: E_rC50 (7d): 50.3 mg/l

Expected to be non harmful/toxic to microorganisms.

Additional information

CLASSIFICATION AND LABELLING

According to the CLP Regulation (EC 1272/2008), acute aquatic toxicity is normally determined using a fish 96-hour LC50 , a crustacean species 48-hour EC50 and/or an algal species 72- or 96-hour EC50; nevertheless it is also specified in the Regulation that data on other species (e.g.Lemna spp.) shall also be considered if the test methodology is suitable. These species cover a range of trophic levels and taxa and are considered as surrogate for all aquatic organisms.

Data are available for Leuciscus idus, Daphnia magna and Lemna minor. Unfortunately, the available data on fish cannot be considered as reliable, thus only data on invertebrates and aquatic plants have been taken into account for the classification purpose.

According to the CLP Regulation (EC 12720/2008) criteria for the identification of the classification categories for hazardous to the aquatic environment, both the invertebrates and aquatic plants studies fixed the short-term effect level in the range of 10 - 100 mg/l.

In conclusion, the substance is classified as Aquatic Chronic 3 (H412) according to the CLP Regulation (EC 1272/2008).