Tris(isopropenyloxy)vinylsilane

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January - February 1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the appropriate OECD test guideline, and in compliance with GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

1981

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

tris(isopropenyloxy)(vinyl)silane

IUPAC Name:

tris(isopropenyloxy)(vinyl)silane

Constituent 2

Reference substance name:

Tris(isopropenyloxy)vinylsilane

EC Number:

239-362-1

EC Name:

Tris(isopropenyloxy)vinylsilane

Cas Number:

15332-99-7

Molecular formula:

C11H18O3Si

IUPAC Name:

[tris(isopropenyloxy)vinyl]silane

Test material form:

other: liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Kleintierfarm Madoerin AG., CH 4414 Fuellinsdorf, Switzerland
- Age at study initiation: males: 8 weeks, females: 10 weeks
- Weight at study initiation: 180-200 g, females: 180-200 g
- Fasting period before study:
- Housing: groups of 5 in Makrolon type-4 cages.
- Diet (e.g. ad libitum): Kliba rat maintenance diet ad libitum.
- Water (e.g. ad libitum): community tap water ad libitum
- Acclimation period: 12 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3°C
- Humidity (%): 30-70%
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12:12

IN-LIFE DATES: From: To:

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose only

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Five levels, 8 ports capable of accepting a variety of Makrolon animal restraint tubes.
- Exposure chamber volume: the internal active volume for exposure of 40 animals by nose only is one litre.
- Method of holding animals in test chamber: Makrolon tubes of appropriate size.
- Source and rate of air: no information
- Method of conditioning air: the air used for aerosol generation was dried by sequential desiccation.
- System of generating particulates/aerosols: The test article was placed in an automatic syringe pump feeding a nebulizer. The test atmosphere generated was diluted with clean air.
- Method of particle size determination: no
- Treatment of exhaust air: no information
- Temperature, humidity, pressure in air chamber: 20.2°C, relative humidity 0 (value obtained by sequential desiccating system), oxygen concentration 20.9%

TEST ATMOSPHERE
- Brief description of analytical method used: sample dissolved in toluene to a final volume of 100 ml, then analysed by gas chromatography.
- Samples taken from breathing zone: yes

TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: no results were reported.

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

4.31 mg/l

No. of animals per sex per dose:

5 males and 5 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: mortality: once per hour during exposure, once after exposure on day 1, twice daily thereafter; clinical signs: once per hour during exposure, once after exposure on day 1, at least once daily thereafter; body weights: Day 1, 8 and 15
- Necropsy of survivors performed: yes - macroscopic changes or abnormalities recorded.
- Other examinations performed: clinical signs.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 4.31 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Remarks on result:

other: The concentrations of the two samples analysed were 4.1 and 4.5 mg/l.

Mortality:

No mortalities occurred.

Clinical signs:

other: There were slight clinical signs in male and female animals on day 1 after the treatment: apathy and piloerection.

Body weight:

There was no treatment-related body weight change.

Gross pathology:

There were no macroscopic findings upon necropsy.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Tris(isopropenyloxy)(vinyl)silane has been tested for acute inhalation toxicity in rat in a study conducted according to OECD 403 and in compliance with GLP. No mortalities occurred, and treatment-related effects were confined to piloerection and apathy on the day after administration, in the five male and five female rats treated with an aerosol containing 4.31 mg/l of the test substance. It is concluded that the LD50 is greater than 4.31 mg/l under the conditions of the test.