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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from database of Japan Ministry of the Environment
Reference:
Composition 0
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material: 2,2,2-Trichloroethane-1,1-diol (Chloral hydrate)
- Molecular formula: C2-H3-Cl3-O2
- Molecular weight: 165.4026g/mol
- Smiles notation: C(C(O)O)(Cl)(Cl)Cl
- InChl: RNFNDJAIBTYOQL-UHFFFAOYSA-N
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Food type: Unicellular green algae(Pseudokirchneriella subcapitata)
- Amount: At a rate of(Daphnia magna) one animal per 0.2 mg Carbon (organic carbon content) / day
Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
21 d
Hardness:
No data
Test temperature:
20±1 deg.C
pH:
No data
Dissolved oxygen:
No data
Salinity:
No data
Nominal and measured concentrations:
6.25,12.5,25.0,50.0, l00 mg / L (common ratio: 2.0) and the control group
Details on test conditions:
TEST SYSTEM
- Renewal rate of test solution (frequency/flow rate):to exchange a total volume of every 48 or 72 hour)
- No. of organisms per vessel: 1 Daphnia magna/container
- No. of vessels per concentration (replicates):10 container /test group
OTHER TEST CONDITIONS
- Photoperiod: Room light, 16-h light / 8 hours dark (bright; AM, 6: 00 ~ PM1O: 00),
- Light intensity: Photon flux density of test near the container: 9-15 µ mol • m-2 • s-1
TEST CONCENTRATIONS
- Test concentrations: 6.25,12.5,25.0,50.0, l00 mg / L (common ratio: 2.0) and the control group
Key result
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
> 96.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
65 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: Breeding inhibition
Remarks on result:
other: 53.8‐81.9mg/L
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
11.5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: survival and litter size
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
23.6 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: cumulative survival production litter size

Tests conducted before FY 2002 needs confirmation of test results, because some of these tests were conducted using a dispersant.

Validity criteria fulfilled:
not specified
Conclusions:
In a Long-term toxicity to aquatic invertebrates study for Chloral hydrate in Daphnia magna when they were exposed for 21 days under semi -static condition, the Median lethal concentration LC50 was found to be >96.2mg/l at inhibition in breeding effect, whereas effect concentration (EC50) were found to be 65 mg/l based on inhibition in breeding (at 95% confidence limit is 53.8 to 81.9 mg/l) while low effect concentration (LOEC) on the cumulative survival production litter size was found to be 23.6 mg/l. And NOEC was found to be 11.5 mg/l based on survival and litter size.
Executive summary:

In a Long-term toxicity to aquatic invertebrates study for Chloral hydrate in Daphnia magna when they were exposed for 21 days under semi -static condition, the Median lethal concentration LC50 was found to be >96.2mg/l at inhibition in breeding effect, whereas effect concentration (EC50) were found to be 65 mg/l based on inhibition in breeding (at 95% confidence limit is 53.8 to 81.9 mg/l) while low effect concentration (LOEC) on the cumulative survival production litter size was found to be 23.6 mg/l. And NOEC was found to be 11.5 mg/l based on survival and litter size.

Considering the NOEC value it is concluded that the test substance Cloral hydrate does not considered for long term aquatic toxicity.

Description of key information

In a Long-term toxicity to aquatic invertebrates study for Chloral hydrate in Daphnia magna when they were exposed for 21 days under semi -static condition, the Median lethal concentration LC50 was found to be >96.2mg/l at inhibition in breeding effect, whereas effect concentration (EC50) were found to be 65 mg/l based on inhibition in breeding (at 95% confidence limit is 53.8 to 81.9 mg/l) while low effect concentration (LOEC) on the cumulative survival production litter size was found to be 23.6 mg/l. And NOEC was found to be 11.5 mg/l based on survival and litter size.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
11.5 mg/L

Additional information

In a Long-term toxicity to aquatic invertebrates study for Chloral hydrate in Daphnia magna when they were exposed for 21 days under semi -static condition, the Median lethal concentration LC50 was found to be >96.2mg/l at inhibition in breeding effect, whereas effect concentration (EC50) were found to be 65 mg/l based on inhibition in breeding (at 95% confidence limit is 53.8 to 81.9 mg/l) while low effect concentration (LOEC) on the cumulative survival production litter size was found to be 23.6 mg/l. And NOEC was found to be 11.5 mg/l based on survival and litter size.

Considering the NOEC value it is concluded that the test substance Cloral hydrate does not considered for long term aquatic toxicity.