Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-992-5 | CAS number: 7783-35-9
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- genetic toxicity in vitro
- Remarks:
- Type of genotoxicity: other: NUCLEIC ACID SYNTHESIS
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- A COMPARATIVE STUDY OF THE EFFECTS OF MERCURY COMPOUNDS ON CELL VIABILITY AND NUCLEIC ACID SYNTHESIS IN HeLa CELLS
- Author:
- EDDIE S-E. CHAO, JOHN F. GIERTHY and GERALD D. FRENKEL
- Year:
- 1 984
- Bibliographic source:
- Biochemical Pharmacology, Vol. 33, No. 12, pp. 1941-1945, 1984
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Genetic toxicity test was performed on HeLa cells
- GLP compliance:
- not specified
- Type of assay:
- other: No data available
Test material
- Reference substance name:
- Mercury sulphate
- EC Number:
- 231-992-5
- EC Name:
- Mercury sulphate
- Cas Number:
- 7783-35-9
- Molecular formula:
- H2O4S.Hg
- IUPAC Name:
- mercury sulfate
- Details on test material:
- - Name of test material: Mercury sulphate
- Molecular formula: H2O4S.Hg
- Molecular weight : 298.67 g/mol
- Smiles notation: [O-]S(=O)(=O)[O-].[Hg+2]
- InChl : InChI=1S/Hg.H2O4S/c;1-5(2,3)4/h;(H2,1,2,3,4)/q+2;/p-2
- Substance type: Solid
- Physical state: Inorganic
- Impurities (identity and concentrations): No data
Constituent 1
Method
- Target gene:
- HeLa cells
Species / strain
- Species / strain / cell type:
- other: HeLa cells
- Details on mammalian cell type (if applicable):
- Details on mammalian cell line
- Type and identity of media: Dulbecco's Modified Eagle's Medium
- Properly maintained: yes
- Periodically checked for Mycoplasma contamination: No data available
- Periodically checked for karyotype stability: No data available
- Periodically "cleansed" against high spontaneous background: No data available - Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- not specified
- Metabolic activation system:
- no data
- Test concentrations with justification for top dose:
- No data available
- Vehicle / solvent:
- No data available
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Remarks:
- no data
- Details on test system and experimental conditions:
- No data available
- Evaluation criteria:
- inhibition concentration
- Statistics:
- No data available
Results and discussion
Test results
- Species / strain:
- other: Hela cells
- Metabolic activation:
- not specified
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- no data
- Remarks on result:
- other: other: Hela cells
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
The genetic toxicity for Mercuric sulfate (7783-35-9) was found to be positive in Hela cells. - Executive summary:
The HeLa cells were grown on monolayer cultures in Dulbecco's Modified Eagle's Medium. Three parameters were tested for study the effect of mercury sulphate as DNA polymerase α was purified from HeLa cells, Nucleic acid synthesis and intact cells. Mercury sulphate and radiolabelled precursor were added and radioactivity was measured after incubation for 30 min at 37°C. Experiment was conducted in duplicates.
Cytotoxicity was also measured in HeLa cells HeLa cells were seeded into
50 mm dishes at a concentration of 360 cells per dish, with 3.6 ml of Dulbecco's Modified Eagle's Medium with 10% heat-inactivated fetal calf serum. After incubation of the cultures at 37°C for 24 hr, 0.4 ml of a series of 2-fold dilutions of a freshly made 2 mM solution of the mercury compound was added to the cultures. After 9 days of further incubation the cultures were washed with 4ml of phosphate buffered saline (pH 7.6), fixed with methanol, and stained with Giemsa, and the number of colonies per dish was determined. Six replicates were done for each concentration.
RNA synthesis in intact cells was inhibited by mercury sulphate.
As the IC 50 value forDNA polymerase α, Intact cells, Isolated Nuclei was found and mercury sulphate as also cytotoxic effect so on the basis of the result it was found thatgenetic toxicity for Mercuric sulfate (7783-35-9) was found to be positive in HeLa cells.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
