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EC number: 218-002-7 | CAS number: 2035-93-0
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The EC50 values with 95 % confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 57.6 (52.2 - 64.4) mg/L and 15.4 (12.2 - 19.2) mg/L, respectively. The EC10 values with 95 % confidence intervals for inhibition of growth rate (ErC10) and yield (EyC10) after 72 hours were 14.7 (11.7 - 16.5) mg/L and <0.316 mg/L, respectively. The NOEC-values for inhibition of growth rate and yield after 72 hours were 3.16 and 1.00 mg/L, respectively.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 57.6 mg/L
- EC10 or NOEC for freshwater algae:
- 14.7 mg/L
Additional information
The toxicity of the test item, 4-Methyl-4-phenylpentan-2-ol, to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to OECD 201 and EC Method C.3. This study is GLP compliant and it has been assigned a reliability score of 1 (reliable with no restrictions).
The study was conducted under static conditions. A stock solution of 100 mg/L was prepared with dilution water and stirred for 15 minutes at 1100 rpm at room temperature with a magnetic stirrer. Six concentrations were tested in a geometrical series with a dilution factor of 3.16 (nominal): 0.316, 1.00, 3.16, 10.0, 31.6, 100 mg/L. Three replicates were tested for each test item concentration and six replicates for the control. The concentrations of the test item in all test concentrations and the control were analytically verified by HPLC-DAD at the start and the end of exposure. The endpoints are based on the nominal test item concentrations.
The EC50 values with 95 % confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 57.6 (52.2 - 64.4) mg/L and 15.4 (12.2 - 19.2) mg/L, respectively. The EC10 values with 95 % confidence intervals for inhibition of growth rate (ErC10) and yield (EyC10) after 72 hours were 14.7 (11.7 - 16.5) mg/L and <0.316 mg/L, respectively. The NOEC-values for inhibition of growth rate and yield after 72 hours were 3.16 and 1.00 mg/L, respectively. The study is considered reliable and relevant for use for this endpoint.
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