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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Data is from review article or handbook

Data source

Reference
Reference Type:
secondary source
Title:
TOXICOLOGICAL EVALUATION
Author:
BG Chemie
Year:
2000
Bibliographic source:
BG Chemie InfoCenter TOXIKOLOGISCHE BEWERTUNGEN, last updated 11/2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Eye irritation study was conducted to test the effects of m chloronitrobenzene on new Zealand rabbits.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chloro-3-nitrobenzene
EC Number:
204-496-1
EC Name:
1-chloro-3-nitrobenzene
Cas Number:
121-73-3
Molecular formula:
C6H4ClNO2
IUPAC Name:
1-chloro-3-nitrobenzene
Test material form:
solid: crystalline
Details on test material:
- Name of test material (as cited in study report): 1-chloro-3-nitrobenzene
- Molecular formula : C6H4ClNO2
- Molecular weight : 157.556 g/mol
- Smiles notation : c1(cc(ccc1)Cl)[N+](=O)[O-]
- Substance Type: Organic
- Physical state: Solid
- Purity 99%
- Impurities (identity and concentrations): No data available

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
No data available

Test system

Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
100 mg dose of the 99% pure chemical
Duration of treatment / exposure:
72hrs
Observation period (in vivo):
72hrs and after 7 days for an reversible effect
Number of animals or in vitro replicates:
3 animals
Details on study design:
TEST SITE
- Area of exposure: conjunctival sac of the left eye

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 72
Remarks on result:
other: Slightly irritating, Hyperaemia of the conjunctival blood vessels,slight cloudiness of the cornea

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
At 1 and 24hr mild to moderate swelling and severe reddening of the conjunctivae occurred. At 48 hours after treatment, one animal showed marked hyperaemia of the conjunctival blood vessels Two animals exhibited slight cloudiness of the cornea. At 72 hours after treatment, 2 animals were still observed to have marked Hyperaemia of the conjunctival blood vessels After 7 days, all signs of irritation were reversible. Therefore, m-chloronitrobenzene was evaluated as slightly irritating to the eye.
Executive summary:

The effects of m-chloronitrobenzene on the eye were studied in 3 New Zealand rabbits which had a single 100 mg dose of the chemical (99% pure) instilled into the conjunctival sac of the left eye. Twenty-four hours prior to the beginning of the experiment, and 72 hours after administration, one drop of sodium fluorescein solution was instilled into the treated eye of each animal, and damage to the cornea was assessed under ultraviolet light. Inspection of the treated eyes at 1, 24, 48 and 72 hours after instillation gave the following results. At 1 and 24 hours after treatment, mild to moderate swelling and severe reddening of the conjunctivae occurred. Two animals exhibited slight cloudiness of the cornea. At 48 hours after treatment, one animal showed marked hyperaemia of the conjunctival blood vessels. The iris of that animal was reddened, while the cornea was slightly clouded. At 72 hours after treatment, 2 animals were still observed to have marked hyperaemia of the conjunctival blood vessels. After 7 days, all signs of irritation were reversible. Therefore, m-chloronitrobenzene was evaluated as slightly irritating to the eye.