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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal
Qualifier:
according to guideline
Guideline:
other: OECD proposal (1979)
Principles of method if other than guideline:
96 hr short term toxicity study was conducted to test the effects of 1Chloro-3-nitrobenzene on fresh water green algae.
GLP compliance:
no
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data available
Analytical monitoring:
not specified
Details on sampling:
- Concentrations: 3.6 mg/l
Vehicle:
yes
Details on test solutions:
DMSO:Dimethyl sulfoxide
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
Stock solutions of the compounds were prepared in dimethylsulfoxide DMSO
Test organisms (species):
Scenedesmus pannonicus
Details on test organisms:
TEST ORGANISM
- Common name: Green algae

Test type:
static
Water media type:
freshwater
Total exposure duration:
14 d
Post exposure observation period:
No data available
Hardness:
No data available
Test temperature:
No data available
pH:
No data available
Dissolved oxygen:
No data available
Salinity:
No data available
Nominal and measured concentrations:
No data available
Details on test conditions:
- Renewal rate of test solution (frequency/flow rate): Daily
Reference substance (positive control):
not specified
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
1.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
No data available
Results with reference substance (positive control):
No data available
Reported statistics and error estimates:
No data available
Validity criteria fulfilled:
not specified
Conclusions:
Based on the effective minimum inhibitory concentration the EC50 was determined to be 3.06mg/l.
Classification may not be done as the study is chronic.
Executive summary:

Chronic toxicity study was carried out for 14 days to test the effects of the test substance on fresh water green algaeScenedesmus pannonicus.

Semistatic conditions were maintained and the renewal of test solution was done daily thereforetheactual LC50 values will be about 85% of the actual concentration used .

Based on the effective minimum inhibitory concentration the EC50 was determined to be 3.06mg/l.

Description of key information

Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical the EC50 was determine to be 15.1 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
15.1 mg/L

Additional information

To evaluate toxicity of aquatic algae, various experimental studies were performed.Based on the experimental data aquired from various sources following is the discription:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100.0 g/L was prepared by dissolving light brown solid sample in DMSO. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.Various concentration were used.With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.The median effective concentration (EC50) for the test substance 1-chloro-3-nitrobenzene, in algae was determined to be 15.1 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous/ non-hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

The above study was further supported by Chronic toxicity study was carried out for 14 days to test the effects of the test substance on fresh water green algae Scenedesmus pannonicus.

Semistatic conditions were maintained and the renewal of test solution was done daily thereforetheactual LC50 values will be about 85% of the actual concentration used .

Based on the effective minimum inhibitory concentration the EC50 was determined to be 3.06 mg/l.

Both the abpve studies were again supported by 96 hr short term toxicity studies which have been carried out to evaluate the effects of 1-Chloro-3-nitrobenzene on Green algaeChlorella pyrenoidosa

Based on the changes in growth the EC50 was determined to be 1.9 mg/l.

Even though varied results for toxicity of aquatic algae were available but, based on the maximum notifiers in ECHA and the effect concentration from first experimental study report,we can consider that the test chemical is toxic and can be classified as aquatic chronic 3.

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