Methyl N-methylanthranilate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

not specified

Analytical monitoring:

not required

Remarks:

Test substance is stable in recommended storage conditions

Vehicle:

not specified

Details on test solutions:

The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 72 hrs continuous stirring. After that analytically detect that stock and set up test concentrations.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.76 cm
- Weight at study initiation (mean and range, SD): average 0.473 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

152.5 mg of CaCO3

Test temperature:

23 °C

pH:

7.7

Dissolved oxygen:

7.6 mg/l

Nominal and measured concentrations:

3.125mg/L,6.25mg/L,12.5mg/L,25mg/L,50mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: LC50 was observed to be to be >12.5 mg/L but <25 mg/L.

Sublethal observations / clinical signs:

8.1. Mortality and symptoms

Nominal	Number of		Mortality (cumulated) after
Concentration fish			3 h				6 h				24h			48h		72h		96h
(mg/l)			V1		V2		V1	V2			V1	V2		V1	V2	V1	V2	V1	V2
Control	4	4	-		-		-	-			-	-		-	-	-	-	-	-
3.125	4	4	-		-		-	-			-	-		-	-	-	-	-	-
6.25	4	4	-		-		-	-			-	-		-	-	-	-	-	-
12.5	4	4	-		-		-	-			-	-		-	-	-	-	-	-
25	4	4	-		-		-	-			4	4		4	4	4	4	4	4
50	4	4	-		-		4	4			4	4		4	4	4	4	4	4

Visible Symptoms:

Nominal	Number of
			3 h			6 h				24h			48h		72h		96h
Concentration		fish	V1	V2		V1	V2		V1		V2		V1	V2	V1	V2	V1	V2
(mg/l)
Control	4	4	NS4 NS4 NS4 NS4 NS4 NS4										NS4 NS4 NS4 NS4 NS4 NS4
3.125	4	4	NS4 NS4 NS4 NS4 NS4 NS4										NS4 NS4 NS4 NS4 NS4 NS4
6.25	4	4	NS4 NS4 M4 M4						M4		M4		NS4 NS4 NS4 NS4 NS4 NS4
12.5	4	4	NS4 NS4 M4				M4		M4		M4		M4	M4	M4	M4	M4	M4
25	4	4	M4	M4		IS4	IS4		-		-		-	-	-	-	-	-
50	4	4	IS4	IS4		-	-		-		-		-	-	-	-	-	-

Explanation of symptoms:

A: apathy

 N: Narcotic-like state

 C: Swimming near water surface

 M: Slow movements compared to control NS: No abnormal symptoms

S: Surfacing behaviour

 L: Loss of equilibrium SM: Shimming movement H1: Hyperactivity

 H2: Hypo activity

 B: Bending behavior

 F: Fin rot

 IS: Invert Swimming

 SB: Swimming behaviour

 RF: Respiratory Function

 P: Pigmentation

8.2. pH and oxygen content:

		Number of	pH readings after
Nominal	fish		24h		48h		72h		96h
Concentration			V1	V2	V1	V2	V1	V2	V1	V2
(mg/l)
Control	4	4	7.68	7.69	8.07	8.08	8.22	8.23	8.29	8.28
3.125	4	4	7.73	7.75	8.09	8.10	8.21	8.22	8.28	8.30
6.25	4	4	7.79	7.81	8.15	8.16	8.23	8.24	8.31	8.34
12.5	4	4	7.85	7.89	8.20	8.21	8.25	8.27	8.36	8.38
25	4	4	-	-	-	-	-	-	-	-
50	4	4	-	-	-	-	-	-	-	-

 

Nominal	Number of		Oxygen content (mg/L) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.1	7.1	6.3	6.3	6.0	6.0	6.0	6.0
3.125	4	4	6.8	6.8	6.4	6.4	5.8	5.8	5.5	5.5
6.25	4	4	6.8	6.8	6.3	6.2	5.8	5.8	5.5	5.5
12.5	4	4	6.8	6.8	6.4	6.4	5.9	5.9	5.4	5.4
25	4	4	-	-	-	-	-	-	-	-
50	4	4	-	-	-	-	-	-	-	-
8.3. Test temperature
Nominal	Number of		Temperature (°C) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	23.5	23.5	22.0	22.0	22.3	22.3	21.6	21.6
3.125	4	4	23.7	23.7	22.3	22.3	22.5	22.5	22.9	22.9
6.25	4	4	23.6	23.6	22.3	22.3	22.5	22.5	22.9	22.9
12.5	4	4	23.6	23.6	22.3	22.3	22.5	22.5	22.9	22.9
25	4	4	-	-	-	-	-	-	-	-
50	4	4	-	-	-	-	-	-	-	-

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. on Zebra Fish Danio rerio was determine to be >12.5 mg/L but <25 mg/L.

Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 72 hrs continuous stirring. After that analytically detect that stock and set up test concentrations of 3.125mg/L,6.25mg/L,12.5mg/L,25mg/L,50mg/L ,respectively . This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >12.5 mg/L but <25 mg/L. Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 72 hrs continuous stirring. After that analytically detect that stock and set up test concentrations of 3.125mg/L,6.25mg/L,12.5mg/L,25mg/L,50mg/L ,respectively .This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be>12.5 mg/L but <25 mg/L.Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

12.5 mg/L

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 72 hrs continuous stirring. After that analytically detect that stock and set up test concentrations of 3.125mg/L,6.25mg/L,12.5mg/L,25mg/L,50mg/L ,respectively .This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be>12.5 mg/L but <25 mg/L.Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.