1-phenyl-3-pyrazolidone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

The main objective of this study is to evaluate the effect of test chemical on the daphnia magna

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

the test solution was prepared by dissolving 500 mg of the test substance in 500.ml of ADaM’s media

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

150.5 mg of CaCO3

Test temperature:

20±2

pH:

7.01

Nominal and measured concentrations:

nominal cocncentration: 6.25 mg/L,12.5 mg/L, 25 mg/L, 50 mg/L, and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Immobility

Nominal Concentration (mg/l)	Number of Daphnids	Immobility
		0 h	24h	48h
Control	10	-	-	-
6.25	10	-	-	5
12.5	10	-	-	10
25	10	-	10	10
50	10	-	10	10
100	10	-	10	10

Ph and oxygen content:

Nominal Concentration (mg/l)	Number of daphnids	pH(mg/L)
		0h	48h
Control	10	7.30	7.40
6.25	10	7.35	7.16
12.5	10	7.39	7.14
25	10	7.41	7.17
50	10	7.45	7.20
100	10	7.48	7.21

Nominal Concentration (mg/l)	Number of daphnids	DO(mg/L)
		0h	48h
Control	10	7.01	6.9
6.25	10	7.09	6.89
12.5	10	7.09	6.89
25	10	7.09	6.89
50	10	7.09	6.86
100	10	7.09	6.85

Test Temperature:

Nominal Concentration (mg/l)	Number of daphnids	Temparature
		0h	48h
Control	10	21.5	20.9
6.25	10	21.5	20.9
12.5	10	21.5	20.9
25	10	21.5	20.9
50	10	21.5	20.9
100	10	21.5	20.9

Validity criteria fulfilled:

yes

Remarks:

1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels

Conclusions:

The median effective concentration of test chemical to daphnia magna was determined to be 6.25 mg/L.

Executive summary:

Acute Immobilization Test of daphnia magna (performed according to OECD Guideline 203) was conducted to assess the toxic effect of test chemical. The test was performed in a static exposure conditions for the period of 48 hours. Test solution was prepared by dissolving the test chemical in the ADaMs medium, and Daphnia magna was treated with different concentrations (6.25, 12.5, 25, 50, and 100 mg/L), along with the control (daphnia exposed without test chemical). Based on the immobilization effect of test chemical to daphnia magna (n=10, per replicate). The median lethal concentration was evaluated. Results conclude that the test chemical has caused the 100% immobilisation of daphnia at all the concentration > 6.25 mg/L. The obtained data from the report determines that EC50 concentration is 6.25 mg/L. From the obtained value the test chemical can be considered to toxic in nature and can be classified into aquatic chronic 2 category.

Description of key information

Acute Immobilization Test of daphnia magna (performed according to OECD Guideline 203) was conducted to assess the toxic effect of test chemical. The test was performed in a static exposure conditions for the period of 48 hours. Test solution was prepared by dissolving the test chemical in the ADaMs medium, and Daphnia magna was treated with different concentrations (6.25, 12.5, 25, 50, and 100 mg/L), along with the control (daphnia exposed without test chemical). Based on the immobilization effect of test chemical to daphnia magna (n=10, per replicate). The median lethal concentration was evaluated. Results conclude that the test chemical has caused the 100% immobilisation of daphnia at all the concentration > 6.25 mg/L. The obtained data from the report determines that EC50 concentration is 6.25 mg/L. From the obtained value the test chemical can be considered to toxic in nature and can be classified into aquatic chronic 2 category.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

6.25 mg/L

Additional information

In the following information from experimental studies report and different peer reviewed journal on short term toxicity testing to aquatic invertabrates for target chemical is summarized:

Acute Immobilization Test of daphnia magna (performed according to OECD Guideline 203) was conducted to assess the toxic effect of test chemical. The test was performed in a static exposure conditions for the period of 48 hours. Test solution was prepared by dissolving the test chemical in the ADaMs medium, and Daphnia magna was treated with different concentrations (6.25, 12.5, 25, 50, and 100 mg/L), along with the control (daphnia exposed without test chemical). Based on the immobilization effect of test chemical to daphnia magna (n=10, per replicate). The median lethal concentration was evaluated. Results conclude that the test chemical has caused the 100% immobilisation of daphnia at all the concentration > 6.25 mg/L. The obtained data from the report determines that EC50 concentration is 6.25 mg/L. From the obtained value the test chemical can be considered to toxic in nature and can be classified into aquatic chronic 2 category.

Short term toxicity test was performed on Daphnia magna for test chemical for 24 hrs under static conditions. No effect on mortality of fish was observed, Therefore, the LC0 value of test chemical to Daphnia magna was determined to be 1 mg/l.

Short term toxicity test was performed on Daphnia magna for 24 hrs under static condition .Lethal concentration to 100% of test organisms was observed, therefore theLC100 of test chemical to Daphnia magna was determined to be 100mg/l.

Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

The stock solution 20.0 mg/L was prepared by dissolving white powder in reconstituted water. The solution was kept in ultrasonic bath for 20 min. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water.0.05,0.10,0.20,0.40,0.80 mg/L concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 0.09 mg/L (95 % CI :0.08-0.11) on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic acute 1 category as per the CLP classification criteria.

Even though varied results for short term toxicity of aquatic invertebrate were available but, based on the maximum notifiers in ECHA and the effect concentration from first experimental study report,we can consider that the test chemical is toxic and can be classified as aquatic chronic 2.