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The acute aquatic toxicity of titanium nitride to fish, invertebrates and algae was determined in accordance with relevant OECD guidelines under static conditions. As titanium nitride is only sparingly soluble, test substance solutions were prepared in accordance with the OECD Series on Testing and Assessment Number 29 (OECD, 2001), and with the general practices for testing of difficult to test substances as outlined in OECD Series of Testing and Assessment Number 23 (OECD, 2000). Substance dissolution was allowed by a seven-day dissolution procedure of the test substance in a pH 6.0 toxicity test medium appropriate for Pimephelas promelas, Daphnia magna and Pseudokirchenriella subcapitata testing, respectively.

In all tests measured titanium concentrations were below the limits of quantification (2 µg/L) at test initiation and at test termination in all control and test exposures. The absence of dissolved titanium in test solutions at test initiation indicates that titanium was not readily leached from titanium nitride during the seven-day exposure period to the toxicity test water. Thus, test results were based on nominal concentrations.

At the maximum tested concentration of 100 mg/L nominal, titanium nitride was neither toxic to P. promelas after 96 h exposure, nor to D. magna or P. subcapitata after 48 h and 72 h exposure, respectively.

Based on the results of these studies, titanium nitride does not need to be classified as toxic to aquatic organisms.

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