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EC number: 807-586-4 | CAS number: 634602-80-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation / corrosion
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study (OECD 431 / 439)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- Version / remarks:
- (April 13, 2004)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Version / remarks:
- (July 22, 2010)
- Deviations:
- no
- Principles of method if other than guideline:
- In addition, the studies follow the test strategy for determination of a corrosion/irritation property as given in the following guideline: OECD Guideline for Testing of Chemicals No. 404, April 24, 2002 (“Acute Dermal Irritation/Corrosion”).
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- (BASF SE, Experimental Toxicology and Ecology, Ludwigshafen, Germany)
Test material
- Reference substance name:
- 1-(2-hydroxyethoxy)-3-[4-(2-methyloctyl)phenoxy]propan-2-ol; 2-(2-hydroxyethoxy)-3-[4-(2-methyloctyl)phenoxy]propan-1-ol
- EC Number:
- 807-586-4
- Cas Number:
- 634602-80-5
- Molecular formula:
- Unspecified
- IUPAC Name:
- 1-(2-hydroxyethoxy)-3-[4-(2-methyloctyl)phenoxy]propan-2-ol; 2-(2-hydroxyethoxy)-3-[4-(2-methyloctyl)phenoxy]propan-1-ol
Constituent 1
Test animals
- Species:
- human
- Strain:
- other: Epi-200, MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia
Test system
- Type of coverage:
- other: The use of a nylon mesh as a spreading support was not necessary for the test substance (determined in a pretest).
- Preparation of test site:
- other: Three dimensional human epidermis model, EpiDerm TM
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: control tissues with: De-ionized water (NC, corrosion), PBS, sterile (NC, irritation), 8-n potassium hydroxide sol. (PC, corrosion) or 5% (w/v) sodium dodecyl sulfate in de-ionized water, sterile (PC, irritation); NC=negative control, PC=positive control;
- Amount / concentration applied:
- Corrosion test: 50 µL of the undiluted test substance or negative and positive control, respectively.
Irritation test: 30 µL of the undiluted test substance or negative and positive control, respectively. - Duration of treatment / exposure:
- Corrossion test: 3 min, 1 h;
Irritation test: 1 h (under the laminar flow hood at room temperature for 25 minutes overall and for 35 minutes in the incubator) - Observation period:
- Additional to the exposure period:
Corrosion test:
Pre-incubation period: 1 - 1.5 h. After exposure (duration see above) the tissues were washed with PBS to remove residual test material after exposure. Rinsed tissues were kept in 24-well plates (holding plates) at room temperature on assay medium until all tissues per application time were dosed and rinsed. The assay medium was then replaced by MTT solution and tissues were incubated for 3 hours.
Irritation test:
Preincubation period: 1 h. After exposure (duration see above) the tissues were washed with PBS to remove residual test material after exposure. Rinsed tissues were blotted on sterile absorbent paper and transferred into new 6-well plates, pre-filled with 0.9 mL fresh medium. When all tissues were rinsed, the surface of each tissue was carefully dried with a sterile cotton swab. Subsequently, the tissues were incubated in the incubator at 37°C for 24 ± 2 hours. After the post-incubation period, the assay medium was replaced by 0.3 mL MTT solution and the tissues were incubated in the incubator for 3 hours. - Number of animals:
- other:
Number of tissues used in the corrosion test: Two tissues per exposure time and test group (test material, NC and PC; 12 tissues per test) were used.
Number of tissues used in the irritation test: Three tissues were treated with the test substance, the PC and NC, respectively. - Details on study design:
- The objective was to assess the potential for corrosive activity and skin irritation of the test material upon first contact with skin. The present test is based on the experience that corrosive and irritant chemicals produce cytotoxicity in human reconstructed epidermis after a short term topical exposure. The test is designed to predict a skin corrosion or irritation potential of a chemical by using the three dimensional human epidermis model EpiDermTM. After application of the test material to the stratum corneum surface of the EpiDermTM tissue the induced cytotoxicity (= loss of viability) is measured by a colorimetric assay. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity. The mitochondrial dehydrogenase reduces the yellow colored water-soluble 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to the insoluble blue colored formazan. After isopropanol-extraction of the formazan from the tissues, the optical density of the extract is determined spectrophotometrically. Optical density of the extracts of test-substance treated tissues is compared to negative control values from tissues and expressed as relative tissue viability.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- other: Viability (% of negativ control), Corrosion test
- Basis:
- mean
- Remarks:
- (2 tissues)
- Time point:
- other: Exposure: 3 min
- Score:
- 100
- Remarks on result:
- other: Negative control
- Irritation parameter:
- other: Viability (% of negativ control), Corrosion test
- Basis:
- mean
- Remarks:
- (2 tissues)
- Time point:
- other: Exposure: 3 min
- Score:
- 112
- Remarks on result:
- other: Test group
- Irritation parameter:
- other: Viability (% of negativ control), Corrosion test
- Basis:
- mean
- Remarks:
- (2 tissues)
- Time point:
- other: Exposure: 3 min
- Score:
- 13
- Remarks on result:
- other: Positive control
- Irritation parameter:
- other: Viability (% of negativ control), Corrosion test
- Basis:
- mean
- Remarks:
- (2 tissues)
- Time point:
- other: Exposure: 1 hour
- Score:
- 100
- Remarks on result:
- other: Negative control
- Irritation parameter:
- other: Viability (% of negativ control), Corrosion test
- Basis:
- mean
- Remarks:
- (2 tissues)
- Time point:
- other: Exposure: 1 h
- Score:
- 91
- Remarks on result:
- other: Test group
- Irritation parameter:
- other: Viability (% of negativ control), Corrosion test
- Basis:
- mean
- Remarks:
- (2 tissues)
- Time point:
- other: Exposure: 1 h
- Score:
- 5
- Remarks on result:
- other: Positive control
- Irritation parameter:
- other: Viability (% of negativ control), Irritation test
- Basis:
- mean
- Remarks:
- (3 tissues)
- Time point:
- other: Exposure: 1 h
- Score:
- 100
- Remarks on result:
- other: Negative control
- Irritation parameter:
- other: Viability (% of negativ control), Irritation test
- Basis:
- mean
- Remarks:
- (3 tissues)
- Time point:
- other: Exposure: 1 h
- Score:
- 3
- Remarks on result:
- other: Test group
- Irritation parameter:
- other: Viability (% of negativ control), Irritation test
- Basis:
- mean
- Remarks:
- (3 tissues)
- Time point:
- other: Exposure: 1 h
- Score:
- 3
- Remarks on result:
- other: Positive control
Applicant's summary and conclusion
- Interpretation of results:
- irritating
- Remarks:
- Migrated information
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