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EC number: 282-490-8
CAS number: 84238-29-9
Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Vetiveria zizanioides, Gramineae.
The test substance does not induce reverse mutation on four Salmonella
typhimurium strains and one Escherichia coli WP2(uvrA-) (pKM 101) strain
according to the OECD Guidelines n°471 and to the method B13/B14 of the
Vetiveria zizanioides, ext. (vetivert
oil) was tested in the Salmonella typhimurium reverse mutation assay
with 4 strains of Salmonella typhimurium (TAI535, TA1537, TA98 and
TA100) and in the Escherichia coli reverse mutation assay with a strain
of Escherichia coli (WP2 uvrA pKM 101). The test was performed in 2
independent experiments in the presence and absence of metabolic
activation S9-mix (Sprague Dawley rat liver S9 induced by Aroclor 1254).
The study procedures described in this report were based on OECD
Guideline 471. In the dose range finding preliminary cytotoxicity test,
Vetiveria zizanioides, ext. was tested at the doses: 50, 150, 500, 1500
and 5000 µg/plate in the strain TA100. There was no evidence of any
bacteriostatic activity in the presence of the test substance. Based on
the results of the dose range finding test, Vetiveria zizanioides, ext.
was tested in all strains, without and with metabolic activation, in
both a plate incorporation and a preincubation assay at 5 dilutions in
paraffin oil (50, 150, 500, 1500, and 5000 µg/plate).
There was no evidence of any increase in
the number of revertant colonies in the presence of the test substance
without and with metabolic activation for all bacterial strains. The
results were confirmed in two separate experiments. There was no
significant difference between the number of spontaneous reversions, the
number of reversions obtained in the positive controls (without and with
metabolic activation), and the mean of corresponding experimental
historic values obtained in the laboratory.
The test substance, Vetiveria
zizanioides, ext., does not induce any mutagenic change in Salmonella
typhimurium TA1535, TA1537, TA98 and TA100 and Escherichia coli WP2
(uvrA- pKM101) without or with metabolic activation according to OECD
Guidelines n° 471 and to the method B13/B14 of the Directive /32/EC.
Based on the results of this study it is concluded that Vetiveria
zizanioides, ext. is not mutagenic in the Salmonella typhimurium reverse
mutation assay and in the Escherichia coli reverse mutation assay.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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