2-amino-4,6-dinitrophenol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

Salmonella Mutagenicity Tests: 111. Results From the Testing of 255 Chemicals

Author:

Errol Zeiger, Beth Anderson, Steve Haworth, Timothy Lawlor, Kristien Mortelmans, and William Speck

Year:

1987

Bibliographic source:

Environmental Mutagenesis Volume 9, Supplement 9:l-110, 1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

Preincubation Assay

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-4-nitrophenol

EC Number:

202-767-9

EC Name:

2-amino-4-nitrophenol

Cas Number:

99-57-0

IUPAC Name:

2-amino-4-nitrophenol

Details on test material:

- Name of test material (as cited in study report): 2-amino-4-nitrophenol
- Substance type: Organic
- Physical state: solid

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA 1535, TA 1537 and TA 100

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

Rat or Hamster liver, S-9, Aroclor 1254 (10%)

Test concentrations with justification for top dose:

33-3333 UG/PLATE

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

9-aminoacridine

sodium azide

other: 2-aminoanthracene

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA 1535, TA 1537 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

TA100

Dose	No Activation  (Negative)		10% HLI  (Negative)		10% RLI  (Negative)
Protocol	Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	61	6.4	93	5.2	80	7.2
33	59	9.8	85	10.1	66	7.5
100	60	2.8	93	6.7	74	3.8
333	59	4.8	93	14.8	71	2.4
1000	51	2.7	85	8.1	85	8.7
3333	27	3.5	42	1.7	28	7.2
Positive Control	1610	77.6	2524	217.1	1430	157.3

TA1535

Dose	No Activation  (Negative)		10% HLI  (Negative)		10% RLI  (Negative)
Protocol	Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	8	1.2	8	1.7	13	0.3
33	6	0.3	11	1.5	11	2.6
100	4	1.7	5	1.9	14	3.5
333	6	1.5	14	1.3	6	1.2
1000	5	0.3	9	2.2	5	1.9
3333	1	0.7	0	0	0	0.3
Positive Control	117	8.4	46	8.4	43	4.2

TA1537

Dose	No Activation  (Negative)		10% HLI  (Negative)		10% RLI  (Negative)
Protocol	Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	13	1.2	16	1.5	16	1.8
33	16	3.4	21	2.8	12	1.2
100	17	4.2	16	0.7	11	1.7
333	16	2.1	17	2.1	12	2.3
1000	14	0.6	18	0.9	10	1.7
3333	6	0.0	11	1.5	7	0.7
Positive Control	303	30.7	338	23.1	167	17.3

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The substance 2-amino-4-nitrophenol gives negative result for mutagenicity in Salmonella typhimurium strains TA 1535, TA 1537 and TA 100 in Ames assay with and without activation.

Executive summary:

The substance 2-amino-4-nitrophenol gives negative result for mutagenicity in Salmonella typhimurium strains TA 1535, TA 1537 and TA 100 in Ames assay with and without activation.