2-amino-4,6-dinitrophenol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from WATER RESOURCES BULLETIN

Data source

Reference

Reference Type:

publication

Title:

Acute Toxicity of Picric Acid and Picramic Acid to Rainbow Trout, Salmo Gairdneri, and American Oyster, Crassostrea Virginica’

Author:

William L. Goodfellow, Jr., Dennis T. Burton, William C. Graves, Lenwood W. Hall, Jr., and Keith R. Cooper

Year:

1983

Bibliographic source:

Water Resources Bulletin, VOL. 19, NO. 4-641-648, AUGUST 1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as below

Principles of method if other than guideline:

Standard Methods (APHA, et al., 1981).

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-4,6-dinitrophenol

EC Number:

202-544-6

EC Name:

2-amino-4,6-dinitrophenol

Cas Number:

96-91-3

Molecular formula:

C6H5N3O5

IUPAC Name:

2-amino-4,6-dinitrophenol

Test material form:

other: Dark red needles

Details on test material:

- Name of test material (as cited in study report): Picramic acid
- Molecular formula (if other than submission substance): C6H5N3O5
- Molecular weight (if other than submission substance): 199.125g\mol
- Smiles notation (if other than submission substance): c1(cc(cc(c1O)N)[N+](=O)[O-])[N+](=O)[O-]
- InChl (if other than submission substance): 1S/C6H5N3O5/c7-4-1-3(8(11)12)2-5(6(4)10)9(13)14/h1-2,10H,7H2
- Substance type: Organic
- Physical state: Dark red needles
- Analytical purity: No data available.
- Lot/batch No.:38C-0117
- Storage condition of test material: The stock solutions were stored in the dark at 5°C.

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

Preparation of the stock solutions was accomplished by saturation of the compound in distilled water and filtration through a GN-6 Metricel 0.45 pm filter. Stock concentrations were determined spectrophotometrically at 237 nm for picramic acid.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout
- Strain: mykiss
- Source: Wytheville National Fish Hatchery, Max Meadows, Virginia.
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): (x = 41.6 _+ 8.7 mm total length
- Weight at study initiation (mean and range, SD): No data available
- Method of breeding: No data available
- Feeding during test- The fish were not fed during the 96-h assay.
- Food type: Not applicable
- Amount: Not applicable
- Frequency: Not applicable

ACCLIMATION
- Acclimation period: 2 weeks
- Acclimation conditions (same as test or not): Yes same as test period.
- Type and amount of food: Fed commercial trout chow daily during acclimation.
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): Less than 3 percent mortality occurred in the laboratory populations of rainbow trout.

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

No data available.

Test conditions

Hardness:

177.0 (+ 11.9) mg/L CaCO3

Test temperature:

10.8 (+0.52)°C

pH:

7.9 (+ 0.04)

Dissolved oxygen:

Not available

Salinity:

Not available

Nominal and measured concentrations:

0, 40, 50, 60, 70, 80, 90, and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 38 l
- Aeration: No aeration is provided.
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable
- Renewal rate of test solution (frequency/flow rate):24 hour
- No. of organisms per vessel: Not sprecified.(25 fish)
- No. of vessels per concentration (replicates): 7 concentration (Two replicates)
- No. of vessels per control (replicates):2
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: No
- Intervals of water quality measurement: Three days

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 12 hlight:12 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations: Analysis of the test concentrations showed that nominal and actual concentrations varied less than 2 percent.
- Test concentrations: 0, 40, 50, 60, 70, 80, 90, and 100 mg/l

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

46.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The picramic acid LC50 values were 90.9 mg/l at 24 h; 68.6 mg/l at 48 h; 56.6 mg/l at 72 h; and 46.2 mg/l at 96 h.
LC05, LC50 and LC 95 were found to be 18.5 46.2 67.8 (2.3-27.6) respectively based on 95 percent confidence limits for acute toxicity.

Applicant's summary and conclusion

Conclusions:

LC50 was found to be 46.2 mg/l for Picramic acid in Oncorhynchus mykiss for 96 hour.

Executive summary:

In Short-term toxicity to fish study for Picramic acid was determined in Oncorhynchus mykiss for 96 hour. The LC50 was found to be 46.2 mg/l for Picramic acid.