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EC number: 211-802-7 | CAS number: 696-99-1
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October - November 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- , corresponds for the most part
- GLP compliance:
- yes
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Details on test solutions:
- Test concentration of activated sludge: 800 mg/L
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Origin: laboratory scale sewage treatment plant unit which runs predominantly with domestic sewage (South Wupper area water authority)
- Pretreatment: none - Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 5 min
- Hardness:
- no data reported.
- Test temperature:
- 20 ± 2°C
- pH:
- ca. 7.7, see tables below.
- Dissolved oxygen:
- see tables below.
- Nominal and measured concentrations:
- - Nominal test concentrations (mg/l): 1000, 1800, 3200, 5600, 10 000
- Test substance concentration in physico-chemical oxygen consumption control (mg/l): 10 000
- Test concentration of the activated sludge (mg/l): 800 - Details on test conditions:
- - Incubation time: 30 min with permanent aeration
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 5 min
- Dose descriptor:
- EC50
- Effect conc.:
- 5 850 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The EC values are determined by statistical methods (probit analysis). Test concentration and reference substance concentration result from the quantities used in the preparation and have not been confirmed by analytical methods.
- Results with reference substance (positive control):
- EC 50 = 15 mg/l
(valid result since the EC50 of the test substance have to be in the range of 5 to 30 mg/l.) - Reported statistics and error estimates:
- No details available.
- Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria of the test method were met.
- Conclusions:
- The study is regarded as a valid guideline study with GLP compliance. The toxicity of the test item to activated sludge gave the result EC50 = 5850 mg/l.
- Executive summary:
The acute toxicity to microorganisms of (benzylamine)trifluoroborn was tested according to EU Method C.11. The method assessed the effect of the test item by measuring the respiration rate under defined conditions in the presence of different test concentrations. The study was conducted under GLP compliance. A mixed population of aquatic microorganisms (activated sludge), taken from a laboratory scale sewage treatment plant unit which runs predominantly with domestic sewage (South Wupper area water authority) was used as inoculum. An incubation period of 30 min was chosen with aeration. Two controls without test item were included in the test design. The sensitivity of the activated sludge was checked using 3.5 -Dichlorophenol as reference compound. The physico-chemical oxygen consumption control was carried out, since some substances may consume oxygen by chemical reactivity. Direct weighings were prepared to give the different test item concentrations, which were 1000, 1800, 3200, 5600 and 10000 mg/L. 3.5 -Dichlorophenol was used in the concentrations of 2.5, 5, 10, 20 and 40 mg/L. 10000 mg/L test item was included in the physico-chemical oxygen consumption control. No analytical methods were applied.
Analysis of the respiration rates at the end of the test gave the following result: EC50 = 5850 mg/L. The EC50 value of the reference compound was measured to be 15 mg/L, thus being in the validity range of 5 - 30 mg/L.
Reference
Test substance:
| Test concentration (mg/l) |
respiratory rate test substance (mg/l.h) |
Phys. Chem. O2-consumption (mg/l.h) |
Respiratory rate - phys. chem. O2-consumption (mg/l.h) |
Inhibition (%) |
| 1 000 | 20.4 | 0.0* | 20.4 | 20.9 |
| 1 800 | 19.0 | 0.0* | 19.0 | 26.4 |
| 3 200 | 16.8 | 0.0* | 16.8 | 34.9 |
| 5 600 | 13.2 | 0.0* | 13.2 | 48.8 |
| 10 000 | 9.6 | 0.0 | 9.6 | 62.8 |
| Control, mean | 25.8 | |||
| Control 1 | 25.2 | |||
| Control 2 | 26.4 |
* The physico chemical oxygen consumption is determined at 10 000 mg/l test substance.
No physico chemical oxygen consumtion has been determined. Therefore lower concentrations of the test substance cause no physico chemical oxygen consumption (deduced values).
Reference substance:
| Test concentration (mg/l) |
respiratory rate (mg/l.h) |
Inhibition (%) |
| 2.5 | 21.6 | 16.3 |
| 5 | 19.5 | 24.4 |
| 10 | 15.6 | 39.5 |
| 20 | 11.0 | 57.4 |
| 40 | 7.2 | 72.1 |
Description of key information
EU Method C.11, GLP, activated sludge from a sewage treatment plant running with domestic sewage
EC50 = 5850 mg/L
EC10 = 463 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 5 850 mg/L
- EC10 or NOEC for microorganisms:
- 463 mg/L
Additional information
The acute toxicity to microorganisms of (benzylamine)trifluoroborn was tested according to EU Method C.11. The method assessed the effect of the test item by measuring the respiration rate under defined conditions in the presence of different test concentrations. The study was conducted under GLP compliance. A mixed population of aquatic microorganisms (activated sludge), taken from a laboratory scale sewage treatment plant unit which runs predominantly with domestic sewage (South Wupper area water authority) was used as inoculum. An incubation period of 30 min was chosen with aeration. Two controls without test item were included in the test design. The sensitivity of the activated sludge was checked using 3.5 -Dichlorophenol as reference compound. The physico-chemical oxygen consumption control was carried out, since some substances may consume oxygen by chemical reactivity. Direct weighings were prepared to give the different test item concentrations, which were 1000, 1800, 3200, 5600 and 10000 mg/L. 3.5 -Dichlorophenol was used in the concentrations of 2.5, 5, 10, 20 and 40 mg/L. 10000 mg/L test item was included in the physico-chemical oxygen consumption control. No analytical methods were applied.
Analysis of the respiration rates at the end of the test gave the following result: EC50 = 5850 mg/L. The EC50 value of the reference compound was measured to be 15 mg/L, thus being in the validity range of 5 - 30 mg/L.
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