Triphenylphosphine oxide

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets national standard methods with accptable restrictions

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Reference 2

Reference Type:

other: recalculation

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: original cited: "Algae inhibition test supported by the UBA (German environmental government agency) " ressembling DIN 38412/9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Triphenylphosphine oxide

EC Number:

212-338-8

EC Name:

Triphenylphosphine oxide

Cas Number:

791-28-6

Molecular formula:

C18H15OP

IUPAC Name:

triphenylphosphine oxide

Details on test material:

- Name of test material (as cited in study report): Triphenylphophinoxid
- Analytical purity: not stated

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: green algae, (Scenedesmus subspicatus)
- Strain: SAG 86.81
ACCLIMATION
Culturing volume: 100 ml

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20 °C

Nominal and measured concentrations:

Nominal concentrations: 0.976, 1.95, 3.9, 7.81, 15.62, 31.25, 62.5, 125 mg/L. A control was tested in paralell.
The stock solution was hardly soluble

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks (nominal volume 250 ml) plugged with gas permeable siliconsponge caps
- Type (delete if not applicable): open
- Initial cells density: Initial count of algae cells/ml: 10000

GROWTH MEDIUM
- Standard medium used: yes; OECD Medium
Temperature: 20 °C
Culturing volume: 10 ml


TEST MEDIUM / WATER PARAMETERS
Test substance preparation: heating
- Culture medium different from test medium: no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

3.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Recalculation (ToxRatPro v2.09)

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

6.04 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Recalculation (ToxRatPro v2.09)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

20.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Recalculation (ToxRatPro v2.09)

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

9.81 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Recalculation (ToxRatPro v2.09)

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

14.366 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Recalculation (ToxRatPro v2.09)

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

29.644 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Recalculation (ToxRatPro v2.09)

Effect concentrations 7

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

7.812 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Recalculation (ToxRatPro v2.09)

Effect concentrations 8

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

3.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Recalculation (ToxRatPro v2.09)

Effect concentrations 9

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

7.599 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: cell denstity

Effect concentrations 10

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

18.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: cell density

Effect concentrations 11

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

56.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: cell density

Details on results:

- Exponential growth in the control: no