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Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from J-CHECK authoritative database
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance Trichloroacetaldehyde.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material: Trichloroacetaldehyde
- Molecular formula: C2HCl3O
- Molecular weight: 147.3879 g/mol
- Physical State: Liquid
- Smiles notation: C(C=O)(Cl)(Cl)Cl
- InChl : 1S/C2HCl3O/c3-2(4,5)1-6/h1H
Oxygen conditions:
not specified
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Concentration of sludge: 30 mg/l
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Parameter followed for biodegradation estimation:
other: BOD and HPLC
Parameter followed for biodegradation estimation:
TOC removal
Details on study design:
Standard type
Key result
Parameter:
other: BOD
Value:
8
Sampling time:
28 d
Remarks on result:
other: Other details not known
Key result
Parameter:
% degradation (TOC removal)
Value:
0
Sampling time:
28 d
Remarks on result:
other: Other details not known
Key result
Parameter:
other: HPLC
Value:
70
Sampling time:
28 d
Remarks on result:
other: Other details not known
Details on results:
Test substance undergoes 8, 0 and 70% degradation by BOD, TOC removal and HPLC parameter in 28 days. The test substance formed Trichloroacetic acid in (Sludge + Test Substance) system.
Validity criteria fulfilled:
not specified
Interpretation of results:
not readily biodegradable
Conclusions:
The percentage degradation of test substance was determined to 8, 0 and 70% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation (8 and 0%), Trichloroacetaldehyde is considered to be not readily biodegradable in water.
Executive summary:

Study was conducted for 28 days for evaluating the percentage biodegradability of test substanceTrichloroacetaldehyde (CAS no. 75-87-6). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to 8, 0 and 70% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation (8 and 0%), Trichloroacetaldehyde is considered to be not readily biodegradable in nature.

Description of key information

Study was conducted for 28 days for evaluating the percentage biodegradability of test substance Trichloroacetaldehyde (CAS no. 75-87-6) (J-CHECK, 2017). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to 8, 0 and 70% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation (8 and 0%), Trichloroacetaldehyde is considered to be not readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Various experimental and predicted data for the target compound Trichloroacetaldehyde(CAS No. 75-87-6) and various supporting studies for its closest read across substance with logKow as the primary descriptor were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental key study, biodegradation experiment was conducted for 28 days for evaluating the test substance Trichloroacetaldehyde (CAS no. 75-87-6). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to 8, 0 and 70% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation (8 and 0%), Trichloroacetaldehyde is considered to be not readily biodegradable in nature.

 

In a prediction using the Estimation Programs Interface Suite (EPI suite, 2017), the biodegradation potential of the test compound Trichloroacetaldehyde(CAS No. 75-87-6) in the presence of mixed populations of environmental microorganisms was estimated.Estimation Programs Interface Suite (EPI suite, 2017) was run to predict the biodegradation potential of the test compound Trichloroacetaldehyde (CAS no. 75 -87 -6) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical  Trichloroacetaldehyde is expected to be not readily biodegradable.

 

In a supporting weight of evidence study from authoritative database and secondary source (HSDB and HPVIS, 2016), for the read across chemical 2,2 -dichloro-1,1,1 -trifluoroethane (CAS no. 306-83-2),biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of read across substance 2,2 -dichloro-1,1,1 –trifluoroethane. The study was performed according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test) at a temperature of 20°C. Activated sludge culture was used as a test inoculum taken from a municipal wastewater treatment facility. Initial test substance conc. used in the study was 12.5 mg/l. DOC concentrations were measured for duplicate samples on days 5, 15 and 28.The percentage degradation of read across substance was determined to be 24% by DOC removal parameter in 28 days. Thus, based on percentage degradation, 2,2 -dichloro-1,1,1 -trifluoroethane is considered to be not readily biodegradable in nature.

 

For the same read across chemical 2,2 -dichloro-1,1,1 -trifluoroethane (CAS no. 306-83-2), biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance 2,2-dichloro-1,1,1-trifluoroethane (J-CHECK, 2017). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to 6 and 4% by BOD and GC parameter in 28 days. Thus, based on percentage degradation, 2,2 -dichloro-1,1,1 -trifluoroethane is considered to be not readily biodegradable in nature.

 

Another biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of read across substance 1,1,1-trichloroethane (CAS no. 71-55-6) (J-CHECK, 2017 and OECD SIDS, 2009). The study was performed according to OECD Guide-line 301 C "Ready Biodegradability: Modified MITI Test (I)" under aerobic conditions. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of read across substance was determined to be 0% by BOD and GC parameter in 14 days. Thus, based on percentage degradation, 1,1,1-trichloroethane is considered to be not readily biodegradable in nature.

 

On the basis of above results for target chemical Trichloroacetaldehyde (from authoritative database J-CHECK and EPI suite, 2017) and for its read across substance (from authoritative database J-CHECK, HSDB and secondary source HPVIS & OECD SIDS), it can be concluded that the test substance Trichloroacetaldehyde can be expected to be not readily biodegradable in nature.