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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from J-CHECK authoritative database
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance
GLP compliance:
not specified
Oxygen conditions:
not specified
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Concentration of sludge: 30 mg/l
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
O2 consumption
Remarks:
BOD
Parameter followed for biodegradation estimation:
test mat. analysis
Remarks:
HPLC
Details on study design:
Standard type
Key result
Parameter:
% degradation (O2 consumption)
Remarks:
BOD
Value:
8
Sampling time:
28 d
Remarks on result:
other: Other details not known
Key result
Parameter:
% degradation (TOC removal)
Value:
0
Sampling time:
28 d
Remarks on result:
other: Other details not known
Key result
Parameter:
% degradation (test mat. analysis)
Remarks:
HPLC
Value:
70
Sampling time:
28 d
Remarks on result:
other: Other details not known
Details on results:
Test substance undergoes 8, 0 and 70% degradation by O2 consumption BOD, TOC removal and test chemical analysis by HPLC parameter respectively in 28 days. The test substance formed Trichloroacetic acid in (Sludge + Test Substance) system.
Validity criteria fulfilled:
not specified
Interpretation of results:
not readily biodegradable
Conclusions:
The percentage degradation of test substance was determined to 8, 0 and 70% by by O2 consumption BOD, TOC removal and test chemical analysis by HPLC parameter respectively in 28 days. Thus, based on percentage degradation (8 and 0%), test chemical is considered to be not readily biodegradable in water.
Executive summary:

Study was conducted for 28 days for evaluating the percentage biodegradability of test substance in this study the concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to 8, 0 and 70% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation (8 and 0%), test chemical is considered to be not readily biodegradable in nature.

Description of key information

Study was conducted for 28 days for evaluating the percentage biodegradability of test substance in this study the concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to 8, 0 and 70% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation (8 and 0%), test chemical is considered to be not readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:
not biodegradable
Type of water:
freshwater

Additional information

Different studies were performed to determine the Biodegradation in water of test chemical and its structurally similar read across chemical and results are summarized below.

In the first study from authoritative database the experiment was conducted for 28 days for evaluating the percentage biodegradability of test substance in this study the concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to 8, 0 and 70% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation (8 and 0%), test chemical is considered to be not readily biodegradable in nature.

 

In next study the Estimation Programs Interface Suite (EPI suite, 2020) was run to predict the biodegradation potential of the test chemical in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical is expected to be not readily biodegradable.

 

To support the above results another study was conducted for 14 days for evaluating the percentage biodegradability of test substance in this study the concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 0 % by O2 consumption (BOD) and test chemical analysis by GC parameter in 14 days. Thus, based on percentage degradation value, test chemical is considered to be not readily biodegradable in nature.

 

By considering the results of all the studies mentioned above it is concluded that test chemical is not readily biodegradable in nature.