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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Biodegradation study was conducted for evaluating the percentage biodegradability of test substance 2,3-dimethylphenol.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): 2,3-dimethylphenol
- Molecular formula (if other than submission substance): C8H10O
- Molecular weight (if other than submission substance): 122.16 g/mol
- Substance type: Organic
- Physical state: Solid
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
No data available.
Duration of test (contact time):
4 d
Initial conc.:
0.016 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
other: % removal
Details on study design:
TEST CONDITIONS
- pH: 8.0
Key result
Parameter:
other: % removal
Value:
99.98
Sampling time:
4 d
Remarks on result:
other: Other details not known
Details on results:
The percentage degradation of test substance was determined to be 99.98% in 4 days.
Validity criteria fulfilled:
not specified
Interpretation of results:
readily biodegradable
Conclusions:
The percentage degradation of test substance was determined to be 99.98% in 4 days. Thus, based on percentage degradation, test chemical 2, 3-Dimethy phenol was considered to be readily biodegradable in water.
Executive summary:

Biodegradation study was conducted for evaluating the percentage biodegradability of test substance 2,3-dimethylphenol (CAS no. 526 -75 -0) under aerobic conditions. Activated sludge was used as a test inoculum. The organic analyses in the study were performed by gas chromatography / mass spectrometry (GC/MS) on methylene chloride extracts of the wastewater samples. Three extracts were collected--a neutral extract, an acid extract, and a base extract. Water samples extracted and analyzed included the raw wastewater after dissolved air flotation (DAF) treatment, effluent from activated sludge (AS) treatment, and effluent from activated carbon (AC) columns.The percentage degradation of test substance was determined to be 99.98% in 4 days. Thus, based on percentage degradation, test chemical 2, 3-Dimethy phenol was considered to be readily biodegradable in nature.

Description of key information

Biodegradation study was conducted for evaluating the percentage biodegradability of test substance 2,3-dimethylphenol (CAS no. 526 -75 -0) under aerobic conditions (E. H. Snider and F. S. Manning, 1982). Activated sludge was used as a test inoculum. The organic analyses in the study were performed by gas chromatography / mass spectrometry (GC/MS) on methylene chloride extracts of the wastewater samples. Three extracts were collected--a neutral extract, an acid extract, and a base extract. Water samples extracted and analyzed included the raw wastewater after dissolved air flotation (DAF) treatment, effluent from activated sludge (AS) treatment, and effluent from activated carbon (AC) columns.The percentage degradation of test substance was determined to be 99.98% in 4 days. Thus, based on percentage degradation, test chemical 2, 3-Dimethy phenol was considered to be readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Various experimental key and supporting studies for the target compound 2,3-Dimethylphenol (CAS No. 526 -75 -0) were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental key study from peer reviewed journal (E. H. Snider and F. S. Manning, 1982) for target chemical2,3-Dimethylphenol(CAS No. 526-75-0), biodegradation experiment was conducted for evaluating the percentage biodegradability of test substance 2,3-dimethylphenol under aerobic conditions. Activated sludge was used as a test inoculum. The organic analyses in the study were performed by gas chromatography / mass spectrometry (GC/MS) on methylene chloride extracts of the wastewater samples. Three extracts were collected--a neutral extract, an acid extract, and a base extract. Water samples extracted and analyzed included the raw wastewater after dissolved air flotation (DAF) treatment, effluent from activated sludge (AS) treatment, and effluent from activated carbon (AC) columns. The percentage degradation of test substance was determined to be 99.98% in 4 days. Thus, based on percentage degradation, test chemical 2, 3-Dimethy phenol was considered to be readily biodegradable in nature.

 

In a supporting biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance 2,3-dimethylphenol (CAS no. 526-75-0) (HSDB, 2016 and SRC PhysProp database, 2017). Activated sludge which is acclimated for a period of 2 days was used as a test inoculum for the study. Initial test substance conc. used for the study was 200 mg/l. The percentage degradation of test substance was determined to be 95.5% degradation by COD removal parameter after 5 days. Thus, based on percentage degradation,2,3-dimethylphenolis considered to be readily biodegradable in nature.

 

Another supporting data from peer reviewed journal (JAMES G. MUELLER et. al, 1991) for the target chemical 2,3 -Dimethylphenol (CAS No. 526-75-0),biodegradation study was carried out for 14 days for evaluating the percentage biodegradability of test substance 2,3 -dimethylphenol. Micro-organism was used as a test inoculum isolated from surface soil which was freshly obtained from the American Creosote Works site. Microbial inoculum was prepared by mixing 25 g of creosote-contaminated surface soil (17) freshly obtained from the American Creosote Works site with 100 ml of 2.5 mM phosphate buffer (pH 7). After being mixed well, the suspension was centrifuged (2,500 rpm, 10 min) to remove larger soil particles. The resultant supernatant was decanted and used as a source of indigenous, "creosote-adapted" microorganisms for the ground water medium (GWM).Ground water medium (GWM) was used for the study. For the biodegradation study, approximately 400 liters of groundwater contaminated with creosote and PCP was recovered from an on-site sampling well. Groundwater was removed from a depth of 7 m through Teflon-coated Bev-a-line tubing (15 mm inner diameter) by means of an electric pump. Groundwater was delivered directly into two freshly rinsed, 208-liter steel drums (DOT-17E) and stored on site for ancillary testing. Five subsamples (1.0 liter) were collected in clean, sterile, 1.0-liter Wheaton bottles fitted with Teflon-lined screw caps and stored on ice for transport to the laboratory. Upon arrival at the laboratory, subsamples were stored in darkness at 2°C for subsequent biodegradation studies, toxicity and teratogenicity testing, and chemical analyses.

It contains 2.5 ml of filtered groundwater (passed through a plug of silanized glass wool to remove undissolved solids) plus 12.5 ml of modified Bushnell-Haas medium. Additionally, two clean, sterile, 1.0-liter Wheaton bottles fitted with Teflon-lined screw caps received 200 ml of the same medium. Ground water contains the test chemical2,3,5-trimethylphenol. Wheaton bottles consist of 200 ml of ground water medium (which contain ground water and modified Bushnell-Haas medium. Duplicate 25 ml samples were immediately extracted for time zero analysis. Flasks were incubated at 30°C with shaking (200 rpm) in the dark for 14 days. Killed-cell controls were prepared for each sampling time point by adding 2.5 ml of a 37% formaldehyde solution to five of the shake flasks containing 25 ml of GWM. After 1, 3, 5, 8, and 14 days of incubation, the entire contents of two active flasks and one killed-cell control flask were separately extracted and analyzed by GC for the presence of creosote constituents. These data were compared with those obtained from untreated (non-inoculated) GWM that had been stored at 2°C during the 14-day incubation period. As the limit of detection of test chemical by GC is very low during a period 14 days, test chemical 2,3-dimethylphenolcan be considered to be readily biodegradable nature.

 

In an additional supporting data from secondary source (P.C. Singer et. al; 1978) for the target chemical 2,3 -Dimethylphenol (CAS No. 526-75-0), preliminary biodegradability screening test was conducted for 20 days for evaluating the percentage biodegradability of test substance 2,3-dimethylphenol. Domestic sewage was used as a test inoculum was obtained from the Chapel Hill sewage treatment plant. The test compound were added to duplicate, acid-washed, 300 ml BOD bottles at concentration of approximately 5 mg/l. Dilution water was prepared from water which had been passed through activated carbon and ion exchange columns and then glass-distilled. Standard nutrients were added to the water as was 0.5 mg/l allylthiourea for control of nitrification. The dilution water was seeded with 1.5 mg/l of domestic sewage. The BOD bottles were filled, stoppered, and incubated in the dark at 65°F for 20 days. Oxygen uptake was measured at various intervals over the 20 day period by means of a Weston and Stack dissolved oxygen meter. The percentage degradation of test substance was determined to be 91% by O₂uptake parameter in 20 days. Thus, based on percentage degradation, test chemical 2,3-dimethylphenol was considered to be readily biodegradable in nature.

 

Another biodegradation study was conducted according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)) for evaluating the percentage biodegradability of test substance 2,3 -dimethylphenol (CAS no. 526 -75 -0) (Kondo, M et. al; 1988). Initial test substance conc. used in the study was 20 mg/l. Namely, a water, acetone or DMSO solution (0.1 ml) of the test chemicals was added to a mixture of river/sea water (4.9 ml) from an unpolluted area and an autoclaved solution (5.0ml) of 0.2% peptone in a sterile test tube with a tight plug. After sealed with film and fixed at an angle of 30°in a dark box, the test tubes were incubated at 30°C and shaked at 120rpm. Inoculum used for the study was mixed culture obtained from different sources (Sea water from Enoshima Beach and River water from Tama River). The percentage degradation of test chemical in river and sea water was determined to be 100 and 54% by BOD parameter in 3 days. Thus, based on percentage degradation, 2,3 -dimethylphenol is considered to be readily biodegradable in nature.

 

On the basis of above results for target chemical 2,3-Dimethylphenol(from peer reviewed journal, authoritative database HSDB and secondary source), it can be concluded that the test substance2,3-Dimethylphenolcan be considered to be readily biodegradable in nature.