Diammonium [[N,N'-ethylenebis[N-(carboxymethyl)glycinato]](4-)-N,N',O,O',ON,ON']manganate(2-)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well documented study without GLP

Data source

Reference

Reference Type:

publication

Title:

Chapter 9, Microbial Degradation of EDTA: New EDTA-degrading Bacterial strains

Author:

Satroutdinov A, Chistyakova T, Dedyukhina E, Minkevich I

Year:

2005

Bibliographic source:

Biogeochemistry of chelating agents (eds Nowack, B. and VanBriesen, J.)

Materials and methods

Principles of method if other than guideline:

EDTA degrading strains were isolated and incubated to EDTA and EDTA complexes with Mg, Ca, Mn, Zn, Co, Cu and Pb

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Edetic acid

EC Number:

200-449-4

EC Name:

Edetic acid

Cas Number:

60-00-4

IUPAC Name:

2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetic acid

Details on test material:

no data available

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Duration of test (contact time):

24 h

Results and discussion

Details on results:

Uncomplexed EDTA and metal-EDTA chelates with low stability constants (log K below 16), such as Mg-, Ca-, Ba-, and Mn-EDTA, were degraded by the washed cell suspensions of both isolates at constant specific rates ranging from 0,310 to 0.525 mmol EDTA/(g cells h). Zn-EDTA with a higher stability constant (log K 18.3) was not completely degraded by resting cell suspensions of Pseudomonas sp, LPM-410 and LPM-4 after an incubation period of 24 h. Initially, the degradation rates of Pseudomonas sp. LPM-410 and LPM-4 were 0.195 and 0.143 mmol EDTA/(g cells h), respectively. After 8 h, degradation rates declined. No degradation of the stable chelates of Pb-, Co-, Cu-EDTA, and Fe(III)-EDTA was observed.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Interpretation of results:

inherently biodegradable

Conclusions:

EDTA-degrading bacterial strains (Pseudomonas sp. LPM-410 and EDTA-dependent strain LPM-4) degraded EDTA and EDTA complexes with Mg, Ca, Mn, and Zn at higher rates (0.143-0.525 mmol EDTA/(g h)), but were unable to degrade EDTA complexes with Co, Cu, and Pb.