Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 304-037-6 | CAS number: 94233-07-5
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well documented study without GLP
Data source
Reference
- Reference Type:
- publication
- Title:
- Chapter 9, Microbial Degradation of EDTA: New EDTA-degrading Bacterial strains
- Author:
- Satroutdinov A, Chistyakova T, Dedyukhina E, Minkevich I
- Year:
- 2 005
- Bibliographic source:
- Biogeochemistry of chelating agents (eds Nowack, B. and VanBriesen, J.)
Materials and methods
- Principles of method if other than guideline:
- EDTA degrading strains were isolated and incubated to EDTA and EDTA complexes with Mg, Ca, Mn, Zn, Co, Cu and Pb
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Edetic acid
- EC Number:
- 200-449-4
- EC Name:
- Edetic acid
- Cas Number:
- 60-00-4
- IUPAC Name:
- 2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetic acid
- Details on test material:
- no data available
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- not specified
- Duration of test (contact time):
- 24 h
Results and discussion
- Details on results:
- Uncomplexed EDTA and metal-EDTA chelates with low stability constants (log K below 16), such as Mg-, Ca-, Ba-, and Mn-EDTA, were degraded by the washed cell suspensions of both isolates at constant specific rates ranging from 0,310 to 0.525 mmol EDTA/(g cells h). Zn-EDTA with a higher stability constant (log K 18.3) was not completely degraded by resting cell suspensions of Pseudomonas sp, LPM-410 and LPM-4 after an incubation period of 24 h. Initially, the degradation rates of Pseudomonas sp. LPM-410 and LPM-4 were 0.195 and 0.143 mmol EDTA/(g cells h), respectively. After 8 h, degradation rates declined. No degradation of the stable chelates of Pb-, Co-, Cu-EDTA, and Fe(III)-EDTA was observed.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- EDTA-degrading bacterial strains (Pseudomonas sp. LPM-410 and EDTA-dependent strain LPM-4) degraded EDTA and EDTA complexes with Mg, Ca, Mn, and Zn at higher rates (0.143-0.525 mmol EDTA/(g h)), but were unable to degrade EDTA complexes with Co, Cu, and Pb.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
