Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report Date:
2016

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)
GLP compliance:
no
Type of method:
shake-flask method to: flask method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Analytical method:
high-performance liquid chromatography

Results and discussion

Partition coefficient
Type:
log Pow
Partition coefficient:
2.69
Temp.:
23 °C
pH:
> 7.8 - < 7.9

Any other information on results incl. tables

Determination stock solution water test substance test substance LogPow

No [ml] [ml] conc. in octanol conc. in water

[mg/L] [mg/L]

A1 5 10 880,6 1,81 2,69

A2 5 10 891,6 1,81 2,69

B1 10 10 882,0 1,82 2,69

B2 10 10 890,0 1,83 2,69

C1 20 10 927,0 1,94 2,68

C2 20 10 910,1 1,85 2,69

mean 2,69

HPLC conditions:

Column : Zorbax SB-C8, 150mm x 4,6mm ID, 5µm,

Eluent A : water + 1 ml/l conc. phosphoric acid

Eluent B : acetonitrile/water 90/10

Gradient : 0 min 10 % B

10 min 100 % B

12 min 100 % B

12,1 min 10 % B

17 min 10% B

Flow rate : 1,5 ml/min

Temperature : 40 °C

Detector : UV, 240 nm

Inj. Volume : 20 μl (water phases) and 6 μl (n-octanol phases)

Recovery rate: 100 - 105%

Applicant's summary and conclusion

Conclusions:
The Log Pow of the substance is 2,69 at 23°C.
Executive summary:

The Partition Coefficient n-octanol/water of the substance was determined according to OECD 107 with the shake flask method and was found to be 2,69 at 23°C.