Reaction mass of methyl N-octadecylterephthalamate and 37437-26-6

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guidelins study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of Methyl N-octadecylterephthala​mate and N,​N'-​Dioctadecylterephtha​lamide

IUPAC Name:

Reaction mass of Methyl N-octadecylterephthala​mate and N,​N'-​Dioctadecylterephtha​lamide

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

A concentration depending turbidity was observed. Despite the attempt to homogenize withe ultra-turrax the solutions remained inhomogenous.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrabärbling
- Source: Zoo Stumpe, Hildesheim
- Length at study initiation (mean): 3,0 cm
- Weight at study initiation (mean): 0,34 g
- Food type: Tetramin Haupfutter, Fa. Tetra

- Acclimation conditions: according to OECD guideline

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

132 mg/l CaCO3

Test temperature:

21-23°C

pH:

7,85-8,12

Dissolved oxygen:

90-99%

Nominal and measured concentrations:

nominal: 100, 180, 320, 580, 1000 mg/l

Details on test conditions:

- Test vessel: Aquarium, 26l
- Volume of medium per vessle: 10l
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality or non-letal effects were observed in any tested concentration (incl. control) at any time point.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In the acute toxicity to fish study with a nominal loading of up to 1000 mg/L of the test item, no effects on Danio rerio were
observed.

Executive summary:

In the acute toxicity test with Danio rerio, the effects of the test item with a nominal loading of 100, 180, 320, 580 and1000 mg/L, were determined according to OECD 203 guideline.

Ten fish were exposed to each concentration and the control.

No analytical monitoring of the test item was carried out.

The effect levels based on the nominal loading of test item are LC50>1000mg/l, NOEC=1000mg/l.

The validity criteria of the test guideline were fulfilled.