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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study under GLP. Only poor documentation available.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Reaction mass of Methyl N-octadecylterephthala​mate and N,​N'-​Dioctadecylterephtha​lamide
IUPAC Name:
Reaction mass of Methyl N-octadecylterephthala​mate and N,​N'-​Dioctadecylterephtha​lamide

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: non-adapted sludge from domestic STP Frankfurt-Sindlingen
- Pretreatment: 5d aeration in nutrient salt solution
- Concentration of sludge: 13 mg/l
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
ca. 152 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
other: not specified

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
ca. 2
Sampling time:
7 d
Parameter:
% degradation (O2 consumption)
Value:
ca. 4
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
ca. 5
Sampling time:
21 d
Parameter:
% degradation (O2 consumption)
Value:
ca. 0
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
The O2 consumption of the reference substance reached >60% within 10d.

Applicant's summary and conclusion

Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The substance is not ready biodegradable under the test conditions.
Executive summary:

The ready biodegradability of the test item was determined in an OECD301F Test with a non-adapted activated sludge over a period of 28 days. The test item was tested at a concentration of 152 mg/L. The biodegradation of the test item was followed by determination of O2 consumption.

In order to check the activity of the test system a reference substance was tested. After 10 days a biodegradation rate of >60% % was reached.

The biodegradation remained in the range of 0 -5 % and the level of 60 % was not reached within the test duration of 28 days.

According to the criteria of the OECD 301F guideline the test item is not readily biodegradable within 28 days.