Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992-10-29 to 1992-10-29
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, no GLP
Cross-reference
Reason / purpose for cross-reference:
read-across source

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Di(isooctadecanoic) acid, diester with oxydi(propanediol)
EC Number:
267-821-6
EC Name:
Di(isooctadecanoic) acid, diester with oxydi(propanediol)
Cas Number:
67938-21-0
Molecular formula:
C42H82O7
IUPAC Name:
di(isooctadecanoic) acid, diester with oxydi(propanediol)
Details on test material:
-Lot E 06209496
-Purity ca. 100%
-Hoechst Code 06-FMFW 155

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
none

Test solutions

Vehicle:
no
Details on test solutions:
-Due to low solubility direct weighing into test medium
-Test concentration 500-1000 mg/L

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Activiated sludge concentration: 1600 mg/L dw.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
none

Test conditions

Test temperature:
20 +/- 2 deg C
pH:
7.2 +/- 0.2
Dissolved oxygen:
saturated
Salinity:
none
Nominal and measured concentrations:
nominal
Details on test conditions:
according OECD Guideline 209
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
No effects on the respiration rate was observed at the highest tested concentration of 1000 mg/L.
Results with reference substance (positive control):
Test concentrations with the reference substance: 5-20-30 mg/L. Result within Guideline range.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The registration substance did not show any effect on the respiration rate of the activated municipal sludge at the highest tested concentration of 1000 mg/L.
Executive summary:

In an OECD 209 Guideline study on the inhibition of the respiration rate of municipal activated sludge no effect was found at the highest tested concentration of 1000 mg/L.