(E)-3-formylbut-2-enyl acetate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982-03-23 (day of application)

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(E)-3-formylbut-2-enyl acetate

EC Number:

247-825-4

EC Name:

(E)-3-formylbut-2-enyl acetate

Cas Number:

26586-02-7

Molecular formula:

C7H10O3

IUPAC Name:

(2E)-3-methyl-4-oxobut-2-en-1-yl acetate

Details on test material:

- Name of test material (as cited in study report): C5-Acetat
- Physical state: liquid
- Analytical purity: no data

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
2 male and 1 female Vienna White rabbits
- Source: Gaukler, 6050 Offenbach, Germany
- Age at study initiation: no data
- Weight at study initiation: 3.82 (animal no. 1; male); 2.70 kg (animal no. 2; male); 2.92 kg (animal no. 2; female)
- Housing: individually in stainless steel cages, area 40 cm x 51 cm
- Diet: ca. 130 g/day; Ovator Solikanin 4 mm; Muskator, Düsseldorf, Germany
- Water: ca. 250 ml/day
- Acclimation period: at least 8 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 26 °C
- Humidity (%): 30 - 70 %
- Air changes (per hr): 15-20
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: no data

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin sites of the test animals

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): ca. 0.5 ml of the unchanged test substance

Duration of treatment / exposure:

1 hour

Observation period:

8 days

Number of animals:

3

Details on study design:

TEST SITE
- Area of exposure: upper third of the back or flanks; a test patch (ca. 2.5 cm x 2.5 cm) was soaked with 0.5 ml of the liquid test substance

REMOVAL OF TEST SUBSTANCE
- Washing (if done): with Lutrol and Lutrol/water (1:1)
- Time after start of exposure: 1 h

SCORING SYSTEM: Draize / OECD
The skin of each animal was read at 30-60 minutes after removal of the application patches as well as at 24 hours, 48 hours, 72 hours, and 8 days after beginning of application.

After sacrifice, the animals were subjected to gross pathology.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Remarks:

of n=3

Time point:

other: 24 - 72 h

Score:

1.56

Max. score:

2

Reversibility:

not fully reversible within: 8 days

Results 2

Irritation parameter:

edema score

Basis:

mean

Remarks:

of n=3

Time point:

other: 24 - 72 h

Score:

0.33

Max. score:

1

Reversibility:

fully reversible

Any other information on results incl. tables

Table: Individual scores and findings

Reading	Animal no.	Erythema	Edema	Symptoms
24 h	1	2	0	R:U
	2	2	0	R:U
	3	2	1	R:U/OE:U
48 h	1	1	0	R:U
	2	1	0	R:U
	3	2	1	R:U/OE:U
72 h	1	1	0	R:U
	2	1	0	R:U
	3	2	1
8 d	1	1	0
	2	1	0	R:U
	3	1	0	S

Abbreviations:

OE:U = edema exceeds the application area

R:U = erythema exceeds the application area

S= desquamation

Applicant's summary and conclusion

Conclusions:

Irritating after 1-hour exposure.