(E)-3-formylbut-2-enyl acetate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1977-08-09

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment; pre-GLP study with restrictions (only one dose level tested, short observation period, limited examinations).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DOT guidelines

Deviations:

yes

Principles of method if other than guideline:

The test was carried out according to DOT guidelines. A definite LD50 was not calculated. No further data.

GLP compliance:

no

Remarks:

pre-GPL study

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(E)-3-formylbut-2-enyl acetate

EC Number:

247-825-4

EC Name:

(E)-3-formylbut-2-enyl acetate

Cas Number:

26586-02-7

Molecular formula:

C7H10O3

IUPAC Name:

(2E)-3-methyl-4-oxobut-2-en-1-yl acetate

Details on test material:

- Name of test material (as cited in study report): beta-formylcrotylacetate (2-methyl-4-acetoxy-butenal)
- Physical state: liquid
- Analytical purity: no data
No further data

Test animals

Species:

rabbit

Strain:

other: albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
5 male and 5 female albino rabbits
- Weight at study initiation: ca. 2 - 3 kg
- Housing: individually in stainless steel cages; size: (57 cm x 44 cm) x 35 cm
- Diet (ad libitum): pelleted standard diet for rabbits
- Acclimation period: at least 3 days
no further data

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 - 23°C
- Humidity (%): 50 - 70%
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: no data

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: spinal region caudal to the ribs
- % coverage: application area; 7 cm x 7 cm (ca. 50 cm²)
- Type of wrap if used: The treated skin site was covered with a vellum-paper, the vellum-paper was fixed with a cohesive tape (Gazofix (TM))
The skin of the back and flanks of each rabbit was shaved at 2 to 16 hours prior to treatment.

REMOVAL OF TEST SUBSTANCE: no data

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 200 µl/kg bw (ca. 212 mg/kg bw; estimated with a density of 1.605 g/ml) of the unchanged test substance

Duration of exposure:

24 hours

Doses:

ca. 212 mg/kg bw (200 µl/kg bw)

No. of animals per sex per dose:

5 males and 5 females (only one dose level tested)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 72 hours
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no

Mortality was determined at 1, 24, 48 , and 72 hour(s) after beginning of application; local signs of irritation and/or toxicity were monitored. Animals that died spontaneously were subjected to a gross post-mortem examination.

Statistics:

no data

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 212 mg/kg bw

Remarks on result:

other: Original value: LD50 > 200 µl/kg bw

Mortality:

No deaths occurred.

Clinical signs:

other: No clinical signs of toxicity were observed. Local signs: Slight, solid strengthening of the skin, partially cracked and fissured was seen at the application site at 5 days after beginning of the application.

Gross pathology:

Pathological examination was not performed since no animal died.

Any other information on results incl. tables

Table: cumulative mortality

Dose	Concentration	Animals	No. of dead animals / no. of exposed animals
			1 h	24 h	48 h	72 h
200 µl /kg bw (ca. 212 mg/kg bw)	Undiluted	Males	0/5	0/5	0/5	0/5
		Females	0/5	0/5	0/5	0/5
		Males + Females	0/10	0/10	0/10	0/10