(E)-3-formylbut-2-enyl acetate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study. Scientifically acceptable.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412, part 9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(E)-3-formylbut-2-enyl acetate

EC Number:

247-825-4

EC Name:

(E)-3-formylbut-2-enyl acetate

Cas Number:

26586-02-7

Molecular formula:

C7H10O3

IUPAC Name:

(2E)-3-methyl-4-oxobut-2-en-1-yl acetate

Details on test material:

- Name of test material (as cited in study report): C5-acetat

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration (nominal 100 mg/l) was prepared. Then, a serial dilution of test medium with culture medium was prepared to obtain the desired test concentrations.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: SAG 86.81
- Source (laboratory, culture collection): Sammlung für Algenkulturen, University of Göttingen

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

20 °C at test end

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: about 10000 cells/ml
- No. of vessels per concentration (replicates): 4 + 2 (without algae)
- No. of vessels per control (replicates): 4 + 2 (without algae)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 201
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:


OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH:
- Photoperiod: continuous white light
- Light intensity and quality:
- Salinity (for marine algae):


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Fluorometric measurement of cell density after 0, 24, 48 and 72 hours.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

1.82 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

5.48 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Results with reference substance (positive control):

Nominal test concentration (mg/l):
0.78, 1.5625, 3.125, 6.25, 12.5, 25, 50, 100, control

Reported statistics and error estimates:

EC50 values were determined using probit analysis.

Any other information on results incl. tables

Cell densities after x hours based on fluorescence values
Nominal test  / Exposure time [h]
concentration /
(mg/l)        /  0 /  24 /  48 /   72 /
Control       / 55 / 185 / 663 / 1515 /
0.78          / 56 / 163 / 616 / 1337 /
1.5625        / 56 / 153 / 591 / 1252 /
3.125         / 55 / 136 / 512 /  758 /
6.25          / 55 / 131 / 400 /  232 /
12.5          / 52 /  96 / 206 /  162 /
25.0          / 53 /  83 / 133 /   79 /
50.0          / 52 /  69 /  78 /   77 /
100.0         / 54 /  48 /  39 /   76 /

% inhibition of growth rate an biomass
            /   72 h /             
     growth rate u / biomass b 
Control       -    /   -       
Solvent       -    /   -       
0.78           4.89 / 11.04     
1.5625         6.74 / 16.29     
3.125         21.34 / 39.47 
6.25          56.70 / 65.25
12.5          65.78 / 82.79     
25.0          88.47 / 91.65 
50.0          87.44 / 96.11
100.0         89.97 / 100.81

EC-values after 72 hours:

biomass
EbC10: < 0.78 mg/l
EbC50: 4.15 mg/l
EbC90: 21.97 mg/l