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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

Biodegradation in water

28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test item aluminium, 2 -(1,3 -dihydro-3 -oxo-5 -sulfo-2H-indol-2 -ylidene)-2,3 -dihydro-3 -oxo-1H-indole-5 -sulfonic acid complex (CAS No.16521 -38 -3)(Experimental study report, 2018). The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. 1 polyseed capsule were added in 500 ml D.I water and then stirred for 1 hour for proper mixing and functioning of inoculum. This gave the bacterial count as 107 to 108 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 78.31%. Degradation of Sodium Benzoate exceeds 13.25% on 7 days & 61.44% on 14th day. The activity of the inoculum is thus verified and the test can be considered as valid. The BOD28 value of test chemical aluminium, 2 -(1,3 -dihydro-3 -oxo-5 -sulfo-2H-indol-2 -ylidene)-2,3 -dihydro-3 -oxo-1H-indole-5 -sulfonic acid complex was observed to be 1.12 mgO2/mg. ThOD was calculated as 1.13 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 99.11%. Based on the results, the test item, under the test conditions, was considered to be readily biodegradable in nature.

Additional information

Biodegradation in water

28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test item aluminium, 2 -(1,3 -dihydro-3 -oxo-5 -sulfo-2H-indol-2 -ylidene)-2,3 -dihydro-3 -oxo-1H-indole-5 -sulfonic acid complex (CAS No.16521 -38 -3)(Experimental study report, 2018). The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. 1 polyseed capsule were added in 500 ml D.I water and then stirred for 1 hour for proper mixing and functioning of inoculum. This gave the bacterial count as 107 to 108 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 78.31%. Degradation of Sodium Benzoate exceeds 13.25% on 7 days & 61.44% on 14th day. The activity of the inoculum is thus verified and the test can be considered as valid. The BOD28 value of test chemical aluminium, 2 -(1,3 -dihydro-3 -oxo-5 -sulfo-2H-indol-2 -ylidene)-2,3 -dihydro-3 -oxo-1H-indole-5 -sulfonic acid complex was observed to be 1.12 mgO2/mg. ThOD was calculated as 1.13 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 99.11%. Based on the results, the test item, under the test conditions, was considered to be readily biodegradable in nature.