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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16.09.2016 – 19.09.2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Data is from ABITEC lab report
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Principles of method if other than guideline:
Study was conducted to determine the toxic nature of chemical by using OECD guidelines.
GLP compliance:
no
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: No data
- Expiration date of the lot/batch: No data
- Purity test date: No data

RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: No data
- Specific activity: No data
- Locations of the label: No data
- Expiration date of radiochemical substance: No data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: No data
- Stability under test conditions: No data
- Solubility and stability of the test substance in the solvent/vehicle: No data
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: No data

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: No data
- Preliminary purification step (if any): No data
- Final dilution of a dissolved solid, stock liquid or gel: No data
- Final preparation of a solid: No data

FORM AS APPLIED IN THE TEST (if different from that of starting material) No data

OTHER SPECIFICS: No
Analytical monitoring:
no
Details on sampling:
No data
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
The solution 100 mg/L was prepared by dissolving the yellow powder in OECD growth medium.

- Controls: OECD medium as specified in OECD 201, used as a control and for dilution of substance.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Age of inoculum (at test initiation): 5x103 cells/ml
- Method of cultivation: No data

ACCLIMATION
- Acclimation period: No data
- Culturing media and conditions (same as test or not): No data
- Any deformed or abnormal cells observed: No data
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Remarks on exposure duration:
±1 hour
Post exposure observation period:
No data
Hardness:
No data
Test temperature:
23°C ± 2°C
pH:
Sample : pH = 8.0 change to pH = 7.5 during the test,
Control : pH = 8.2 changed to pH = 7.8 during the test
Dissolved oxygen:
No data
Salinity:
No data
Conductivity:
No data
Nominal and measured concentrations:
No data
Details on test conditions:
TEST SYSTEM
- Test vessel: 50ml glass vessel
- Type : closed (with air-permeable stopper)
- Material, size, headspace, fill volume: 7.5 ml
- Aeration: No data
- Type of flow-through (e.g. peristaltic or proportional diluter): No data
- Renewal rate of test solution (frequency/flow rate): No data
- Initial cells density: 5x103 cells/mL
- Control end cells density: No data
- No. of organisms per vessel: No data
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): No data
- No. of vessels per vehicle control (replicates): No data

GROWTH MEDIUM
- Standard medium used: Yes

OTHER TEST CONDITIONS
- Adjustment of pH: Without adjustment
- Photoperiod: Continuous
- Light intensity and quality: 6000 lx - 8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell counting: Electronic particle counter.
- Chlorophyll measurement: No
- Other: EC50 was calculated
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Statistics
Remarks:
No significant difference p = 0.060
Details on results:
No data
Results with reference substance (positive control):
- Results with reference substance valid
- ErC50: 0.69 mg/L K2Cr2O7
Reported statistics and error estimates:
The differences in means of control and sample were estimated by the test t-test for independent groups at a 95% confidence level, all individual replicates were used. Statistically significant differences are for p < 0.05.
Validity criteria fulfilled:
yes
Conclusions:
The median effective concentration (EC50) for the test substance, Sodium 2-(2,3-dihydro-1,3-dioxo-1H-inden-2-yl)quinoline-6-sulphonate, in a freshwater algae Desmodesmus subspicatus was determined to be >100 mg/L on the basis of effects on growth rate in a 72 hour study.
Executive summary:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Sodium 2-(2,3-dihydro-1,3-dioxo-1H- inden-2-yl) quinoline-6-sulphonate according to OECD Guideline 201.

A limit test at sample concentration of 100 mg/L was performed. Effects on the growth rate of the organism were studied.

The median effective concentration (EC50) for the test substance, Sodium 2-(2,3-dihydro-1,3-dioxo-1H-inden-2-yl)quinoline-6-sulphonate, in Desmodesmus subspicatus was determined to be >100 mg/L. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance Sodium 2-(2,3-dihydro-1,3-dioxo-1H-inden-2-yl)quinoline-6-sulphonate does not exhibit toxicity to aquatic algae (Desmodesmus subspicatus).

Description of key information

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Sodium 2-(2,3-dihydro-1,3-dioxo-1H- inden-2-yl) quinoline-6-sulphonate according to OECD Guideline 201. A limit test at sample concentration of 100 mg/L was performed. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance, Sodium 2-(2,3-dihydro-1,3-dioxo-1H-inden-2-yl)quinoline-6-sulphonate, in Desmodesmus subspicatus was determined to be >100 mg/L. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance Sodium 2-(2,3-dihydro-1,3-dioxo-1H-inden-2-yl)quinoline-6-sulphonate does not exhibit toxicity to aquatic algae (Desmodesmus subspicatus), and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L

Additional information

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Sodium 2-(2,3-dihydro-1,3-dioxo-1H- inden-2-yl) quinoline-6-sulphonate according to OECD Guideline 201. A limit test at sample concentration of 100 mg/L was performed. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance, Sodium 2-(2,3-dihydro-1,3-dioxo-1H-inden-2-yl)quinoline-6-sulphonate, in Desmodesmus subspicatus was determined to be >100 mg/L. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance Sodium 2-(2,3-dihydro-1,3-dioxo-1H-inden-2-yl)quinoline-6-sulphonate does not exhibit toxicity to aquatic algae (Desmodesmus subspicatus), and not classified as per the CLP classification criteria.