Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Data is from UERL lab
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
This study was designed to assess the toxic effects of the test compound on the Zebra Fish Danio rerio .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
GLP compliance:
yes
Analytical monitoring:
not specified
Details on sampling:
- Sample storage conditions before analysis: Ambient Temp (23 to 27 °C)
Vehicle:
yes
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Age at study initiation (mean and range, SD):3-4 months
- Length at study initiation (length definition, mean, range and SD): average 3.42 cm
- Weight at study initiation (mean and range, SD): average 0.335 g
- Method of breeding:
- Feeding during test
- Food type:Standard brand feed
- Frequency: once daily
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
148.5
Test temperature:
25°C
pH:
7.01
Dissolved oxygen:
7.06 (mg/l)
Nominal and measured concentrations:
100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: all plastic aquaria having the water capacity of 5 liters
- Type (delete if not applicable): No data available
- Material, size, headspace, fill volume: No data available
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: 8 fishes
- No. of vessels per concentration (replicates): No data available
- No. of vessels per control (replicates): 8 fishes
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductivity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available

OTHER TEST CONDITIONS
- Adjustment of pH: No data available
- Photoperiod: 12 hours light and 12-hour darkness
- Light intensity: No data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality and visible symptoms

TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study: A Limit test was conducted with the test concentrations of 100 mg/L. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24, 48, 72 and 96 hours of the start of the experiment.
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Nontoxic
Details on results:
- Behavioural abnormalities: apathy, Narcotic-like state, Swimming near water surface, Slow movements compared to control, Surfacing behaviour, Loss of equilibrium, Swimming movement, Hyperactivity, Hypoactivity, Bending behavior, Fin rot, Invert Swimming, Swimming behaviour, Respiratory Function , Pigmentation
- Observations on body length and weight: No data available
- Other biological observations: No data available
- Mortality of control: No mortality was noted in the control fishes
- Other adverse effects control: No data available
- Abnormal responses: No data available
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No data available
- Effect concentrations exceeding solubility of substance in test medium: No data available
Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 29556-33-0on Zebra Fish Danio rerio was found to be >100mg/L.
Executive summary:

This study was designed to assess the toxic effects of the test compound on the Zebra Fish Danio rerio.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. A 96 h study was conducted for CAS No.29556-33-0 on Zebra Fish Danio rerio, at a test concentrations of 100 mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be >100mg/L.

Description of key information

A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. A 96 h study was conducted for CAS No.29556-33-0 on Zebra Fish Danio rerio, at a test concentrations of 100 mg/L. The lethal concentrations LC50 was determine to be >100mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

This study was designed to assess the toxic effects of the test compound on the Zebra FishDanio rerio.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. A 96 h study was conducted for CAS No.29556-33-0 on Zebra Fish Danio rerio, at a test concentrations of 100 mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was determine to be >100mg/L.