Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 233-520-3 | CAS number: 10213-78-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 18-12-2009 - 24-4-2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, well conducted. No major restrictions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,2'-(octadec-9-enylimino)bisethanol
- EC Number:
- 246-807-3
- EC Name:
- 2,2'-(octadec-9-enylimino)bisethanol
- Cas Number:
- 25307-17-9
- Molecular formula:
- C22H45NO2
- IUPAC Name:
- 2,2'-(Octadec-9-enylimino)bisethanol
- Test material form:
- liquid
- Details on test material:
- Chemical name : 2,2'-(octadec-9-enylimino)bisethanol
EC number : 246-807-3
Based on the qualitative and quantitative information on the composition, the sample used are representative of the boundary composition shared and agree by each registrant.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Storage of samples: samples were analysed immediately after sampling without storage.
Samples treatment: samples were diluted in methanol.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution 5 mg/L CECAJEL 210 at was prepared on the day when the test was started and then on each day the solutions were renewed, Ie. after 24, 48 and 72 hours. Solutions were prepared by mixing 10 mg of the test item in 2 litres of dilution water and diluted in order to achieve the
desired range of concentrations.
The appearance of the test solutions was visually checked at the beginning and at the end of the test. Solutions were found to be clear, colourless \
over the period of the test. No precipitation was observed at the end of the test
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- The organisms used for the test were Dania rerio (Teleostei, Cyprinidae). Batch n° 09/Br/02/1 was supplied by Elevage de la Grande Riviere
(69490 Saint-Forgeux). The fish were placed under the test conditions (medium, identical or lower biological loading, temperature, lighting, etc.) 20
days before the beginning of the test. The fish selected for the test were all from the same batch and were of the same age (date of birth stated by the supplier: 20/08/08). Their size (determined by measuring 10 randomly sampled fish) was 3.2 ± 0.18 cm. The mortality in the aquarium containing
the batch of fish used for the tests was less than 5 % (observed mortality: 0 %) over the 7 days preceding the test.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 22.4 ± 0.5 °C
- pH:
- 7.43-8.05
- Dissolved oxygen:
- Did not fall below 77 % saturation.
- Nominal and measured concentrations:
- 0.500, 0.370, 0.274, 0.203 and 0.151 mg/L (Nominal)
- Details on test conditions:
- The study was carried out in a thermostatically controlled room. The temperature, which was measured on a continuous basis in a test flask, was 22.4 ± 0.5 °C. The room was equipped with a programmed lighting system which produced a controlled lighting sequence: intensity between 400 and
800 lux at the surface of the aquaria; photo-period of 12 hours of darkness per 24 h. Physico-chemical parameters were measured using a
METTLER TOLEDO 345 pH-meter for measurement of pH and with a WTW OXI 538 oxymeter for dissolved oxygen measurement. The study was
performed using aquariums with a nominal capacity of 5 Iitres, covered with Altuglas®cover plates drilled with holes to allow the test solutions to be
aerated. Volume of test medium per aquarium was 2.5 L.
At the beginning of the test, appropriate volumes of the stock solution of CECAJEL 210 (nominal concentration 5 mg/L) were poured directly into the test aquarium, which were topped up with dilution water to obtain the above mentioned range of nominal concentrations (without pH-adjustment).
Number of fish :10 for each test concentration and for the control (2 replicates). The definitive test was carried out under semi-static conditions.
Five fish were added in each test aquarium which were covered and incubated. Two aquaria were prepared for each
concentration. The fish were examined after 1 hour and at intervals not exceeding 24 hours. They were considered to be dead if no reaction was
obtained when their caudal peduncle was stimulated and if no respiratory movement was observed. The visible anomalies were noted,
as were any sub-lethal effects such as loss of balance, pigmentation, changes in swimming behaviour, respiratory distress, etc.
Solutions were renewed after 24, 48 and 72 hours and physical parameters were measured daily. - Reference substance (positive control):
- yes
- Remarks:
- Conducted on every fish batch
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.29 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.25 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95 %(0.24-0.28)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.14 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% (0.11-0.18)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: ND
- Details on results:
- The test chemical displays significant toxicity to fish.
- Results with reference substance (positive control):
- The sensitivity of the test organisms was checked at least once for each new batch of fish, by determining the LC50-24h of the potassium
dichromate. The results of the last test, carried out on 12/03/09, indicated a LC50-24h of 250 mglL. For reference, the former AFNOR standard
NF T 90-303 states that the LC5Q-24h of thepotassium dichromate with respect to Dania rerio must be between 200 and 400 mglL. The fish batch
was considered acceptable for testing. - Reported statistics and error estimates:
- The mortality data are analyzed using an Excel sheet program. It was designed to calculate the LC50 value and the 95% confidence interval. Probit
analysis is generally used to calculate the 24-, 48-, 72 and 96-hour LC50 values.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Percentage Mortality
Nominal mg/L Mean Measured mg/L 24 48 72 96 0.50 0.29 0 70 100 100 0.37 0.22 0 30 90 100 0.27 0.14 0 0 30 60 0.20 0.09 0 0 10 20 0.15 0.05 0 0 0 10 Control - 0 0 0 0
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- This study can be considered reliable without restriction but the identity of the test substance has been clarified.
- Executive summary:
Test well conducted with in general with validated analytical method ,GLP accreditation and analytical certificate. CAS number is contradictory will need clarifying. May be a toxicity overestmation for risk assesment purposes. Suitable for classification and labeling. Some minor discrepancies. Can be considered reliable without significant restrictions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.