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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
None
GLP compliance:
yes
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
Not available
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
The test medium used had the following composition :
stock solution concentration in test
NaHC03 65 mg/1 MgS0 4 : 65 mg/1
MgS0 4 * 7 H 2 0 123.3 mg/1
KCI 5.8 mg/1
CaCI 2 * 2 H20 294 mg/1
Na 2 Si03 *9H2O 10 mg/1
NaN03 274 ug/L
KH 2 PO4 143 ug/L
K 2 HP0 4 184 ug/L
The test water medium was prepared with bidistilled water
The pH was in a range of 7.8- 8.2
Test No. : G 171 14
The medium was oxygen saturated at the beginning of the test.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Method of breeding: Cultures of Daphnia magna are maintained in glass vessels containing aprox. 3.0 L of reconstituted water (see test medium) at 18 - 22 °C. The water was renewed partially three times weekly. The daphnids were feed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food was consumed within 24h.
- Pretreatment: 24 hours before the start of the exposure reproductive daphnids were separated from the young daphnids by sieving all individuals through a 710 µm sieve.
The quality of the daphnids is checked at least every 3 months by determining the EC50 values (24 h) for potassium dichromate (range: 0.8- 1.5 mg/l). For each concentration, of test substance, 20 daphnids divided in two groups of 10 animals are used.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
Test evaluations were done according to the guidelines by visual assessment of mobility of the daphnids after 24 and 48 hours.
Hardness:
136 mg/L CaC03
Test temperature:
21 °C
pH:
7.8- 8.2
Dissolved oxygen:
Not available
Salinity:
0.8 mmol/L
Nominal and measured concentrations:
Based on a pretest, the test concentrations were chosen as 58/100 and 180 mg/L nominal.
Details on test conditions:
- Ca/mg ratio: 4 : 1
- Alkalinity: 0.8 mmol/L
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
>= 8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
In the control and in all test concentrations no immobilization of the Daphnia was observed.
Results with reference substance (positive control):
Potassium dichromate ( EC50: 1.01 mg/L )
Reported statistics and error estimates:
None

Immobilization of daphnids:


 













































































Nominal Conc. Of test material (mg/l)No. of animalsno. of responses% immobility
24 h48 h24 h48 h
Blank100000
100000
58100000
100000
100100000
100000
180100000
100000
Validity criteria fulfilled:
yes
Conclusions:
The EC50 and EC100 values are clearly higher than 8 mg/L after 48 hours. The EC50 values could not be calculated.
Executive summary:

Short-term toxicity of FAT 36152/G to Daphnia magna was determined in a 48-hours static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4 April 84, and EEC Directive 841449, L 251, Part C 2. The test was performed in compliance with the Good Laboratory Practice Regulations. The nominal concentrations tested were 58/100 and 180 mg/L, and a control without any additions. The test concentrations were prepared from the supernatant of a 0.5 g/0.5 l stock solution, in which most of the substance was settled down. For the preparation of the test concentrations only the supernatant of the stock solution was taken. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of <3.4 - 15.0 % of the nominal values. In the control and in all test concentrations no immobilization of the Daphnia was observed. The EC0 value is at least 8 mg/l, but might even be higher than this concentration, the EC50 and EC100 values are clearly higher than 8 mg/l after 48 hours. Therefore, the EC50 values could not be calculated.


 


Results: (24h & 48h)


EC0: >8 mg/l


EC50: >8 mg/l


EC100:>8 mg/l


NOEC: ≥8 mg/l

Description of key information

The EC50 and EC100 values are  higher than 8 mg/l after 48 hours. 

Key value for chemical safety assessment

Additional information

Short-term toxicity of FAT 36152/G to Daphnia magna was determined in a 48-hours static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4 April 84, and EEC Directive 841449, L 251, Part C 2. The nominal concentrations tested were 58/100 and 180 mg/L. During test duration the test article concentrations in the analysed test media were in the range of <3.4 - 15.0 % of the nominal values, hence the results were reported in terms of measured concentrations. In the control and in all test concentrations no immobilization of the Daphnia was observed. Therefore, the EC0, EC50 and EC100 values for 48-hours exposure duration were considered to be >8 mg/l


 


In another supporting study, short-term toxicity of FAT 36152/D to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 84. The nominal concentrations tested were 0.04, 0.20, 0.40, 2.0 and 4.0 mg test substance/L, a solvent control group and a control without any additions. In the control no immobility or mortality of the test animals was observed. FAT 36152/D had no acute toxic effect on Daphnia magna during the test duration of 48 hours up to the highest concentration tested of 4.0 mg test substance/l (as the highest concentration that was possible to dissolve in dimethylformamid p.A. at room temperature and an addition of 100 µL solvent stock solution to 1 L test medium). Therefore, the NOEC (highest concentrationtested without toxic effect) of FAT 36152/D was determined to be 4.0 mg/L or higher.