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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian germ cell study: gene mutation
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed publication

Data source

Reference
Reference Type:
publication
Title:
Study of artificial flavouring substances for mutagenicity in the Salmonella/microsome, Basc and micronucleus tests
Author:
D. Wild, M.-T. King, E. Gocke and K. Eckhardt
Bibliographic source:
Fd Chem. Toxic. Vol. 21, No. 6, pp. 707-719, 1983

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Sex linked recessive lethal mutation assay (Wuergler et al., 1977)
Principles of method if other than guideline:
gene mutation in vivo is studied for the test compound 2 Phenylpropionaldehyde
GLP compliance:
not specified
Type of assay:
Drosophila SLRL assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2 Phenylpropionaldehyde
- Molecular formula (if other than submission substance): C9-H10-O
- Molecular weight (if other than submission substance): 134.177 g/mol
- Substance type: organic
- Physical state: No data available
Purity: No data available
- Impurities (identity and concentrations): No data available

Test animals

Species:
Drosophila melanogaster
Strain:
other: Berlin K (wild-type) and Basc strains
Sex:
male

Administration / exposure

Route of administration:
oral: feed
Vehicle:
Solutions or emulsions of test substances to be fed to the flies were prepared in 5% saccharose, with the addition of 2% ethanol and 2% Tween 80 for compounds that were poorly soluble in water.
Details on exposure:
No data available
Duration of treatment / exposure:
No data available
Frequency of treatment:
No data available
Post exposure period:
No data available
Doses / concentrations
Remarks:
Doses / Concentrations:
25mM
Basis:

No. of animals per sex per dose:
No data available
Control animals:
not specified
Positive control(s):
No data available

Examinations

Tissues and cell types examined:
No data available
Details of tissue and slide preparation:
No data available
Evaluation criteria:
SLRL mutation
Statistics:
Statistical significance determined by methods of Kastenbaum and Bowman (1970).

Results and discussion

Test results
Sex:
male
Genotoxicity:
negative
Toxicity:
not specified
Vehicle controls validity:
not specified
Negative controls validity:
not specified
Positive controls validity:
not specified

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative
The given test material 2 Phenylpropionaldehyde does not induce gene mutation in vivo in Drosophila melanogaster
Executive summary:

The Basc test on Drosophila was performed to study the gene mutation ability of2 Phenylpropionaldehyde.

 

Solutions or emulsions of test substances to be fed to the flies were prepared in 5% saccharose, with the addition of 2% ethanol and 2% Tween 80 for compounds that were poorly soluble in water.

 

The given test material2 Phenylpropionaldehydedoes not induce gene mutation in vivo in Drosophila melanogaster.