Diphosphoric acid, zinc salt, compd. with 1,3,5-triazine-2,4,6-triamine (1:1:2)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted to OECD guidelines and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of 1,3,5-triazine-2,4,6-triamine and zinc bis(dihydrogen phosphate)

EC Number:

690-512-6

Cas Number:

1271172-98-5

Molecular formula:

Zn(C3H7N6)2(P2O7)

IUPAC Name:

Reaction products of 1,3,5-triazine-2,4,6-triamine and zinc bis(dihydrogen phosphate)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Main test:
- Concentrations: 0, 10, 18, 32, 56 and 100 mg/L (nominal).
- Frequency: Samples for analysis were taken from each concentration and the control at 0 and 48 hours (start and end of study).
- Sampling method: To each sample, 100 uL KCl (10% in water) and 100 uL HCl conc. were added. For the concentration of 10 and 18 mg/L and the control the mixture was filled up to 10 mL with test solution and control medium respectivey. For the concentration 100 mg/L, 56 mg/L and 32 mg/L a dilution ratio of 1:10 1:5 and 1:2 respectively was used.
- Sample storage conditions before analysis: Not detailed, but assumed to be analysed on the day of sampling.

Test solutions

Vehicle:

yes

Details on test solutions:

A saturated solution of test material was prepared by weighing nominal loading rates, adding the corresponding amount of water with subsequent vigorous shaking for 24 hours. The resulting saturated solutions were then filtered using 0.45 uM filters. The technique essentially provided a water solubel fraction of nominal loading rates, further characterised by chemical analysis.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna straus
- Strain: Berlin
- Source: Umweltbundesamt Berlin
- Age at study initiation: < 24 hours old
- Method of breeding: Parthenogenesis
- Feeding during test: None

ACCLIMATION
- Acclimation period: Daphnia magna were maintained continuously for acute toxicity tests
- Acclimation conditions (same as test or not): Same as test (except no light in tests)
- Type and amount of food: Unicellular green algae (Desmodesmus Subspicatus)
- Health during acclimation (any mortality observed): Neonate Daphnia were allowed a 30 minute settling in period before addition to test media. Animals which showed apparent damage were not used for tests.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Hardness of dilution water was measured as 250 mg CaCO3/L

Test temperature:

18.1 to 18.4°C

pH:

6.7 to 7.6

Dissolved oxygen:

The concentration of dissolved oxygen stayed above 8.1 mg/L throughout the test

Nominal and measured concentrations:

Nominal: 0, 10, 18, 32, 56 and 100 mg/L
Measured (calculated from experimentally determined Zn value based on the known composition; geometric mean of 24 and 48 hour results): 4.4, 8.1, 13.6, 24.4 and 42.3 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers
- Type: open
- Material, size, headspace, fill volume: 50 mL (tall shape) and filled with 20 mL control or test media.
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4, reconstituted water (including appropriate vitamins).
- Intervals of water quality measurement: Daily for temperature, and at 0 and 48 hours for pH and oxygen concentration.

OTHER TEST CONDITIONS
- Photoperiod: No light (test conducted in darkness)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility at 24 and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 1.8
- Test concentrations: 0, 10, 18, 32, 56 and 100 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 42 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

element

Remarks:

;calculated based on measure Zn content (Zn content in test material known)

Basis for effect:

mobility

Remarks on result:

other: Greater than limit of solubility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

24 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

element

Remarks:

;calculated based on measured Zn content (Zn content in test material known)

Basis for effect:

mobility

Remarks on result:

other: Not determinable

Details on results:

- The EC50 value exceeded solubility of substance in test medium: Due to the low solubility of the test substance, concentration levels that would provide an EC50 value to Daphnia could not be reached. The effect concentration is greater than the limit of solubility (> 42 mg/L).

As effects were observed at a nominal concentration of 100 mg/L (measured ca. 42 mg/L), the NOEC was determined to be 56 mg/L (measured ca. 24 mg/L).

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50: 1.7 mg/L. Within historical range.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The geometric mean exposure concentration for samples taken over the 48 hour period was 42.3 mg/L (based on Zn analysis).
No immobility or abnormal behaviour was noted at any point in control vessels. In test vessels, 45% immobility was observed at measured concentrations of 42.3 mg/L. The concentration causing 50% immobility could not be determined and was detailed as > 42 mg/L.