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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, test procedure in accordance with official guideline, meets generally accepted scientific principles, acceptable for assessment.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
GLP compliance:
not specified
Analytical monitoring:
not specified
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna Straus were from the Stamm Berlin.
Test type:
not specified
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Test temperature:
19 - 21 °C
pH:
8.2
Dissolved oxygen:
Oxygen content 48h: 7.8 mg/l at 0 and 48 h
Salinity:
Molar ratio Na:K = 11.2 : 3.2
Nominal and measured concentrations:
0 and 125 mg/l
Details on test conditions:
Test vessel: Centrifuge tubes with a flattened bottomTest volume: 50 ml
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 125 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
yes
Conclusions:
The results of the test showed a EC50 48 h > 125 mg/l.
Executive summary:

The result of the acute toxicity to Daphnia magna was performed according to OECD 202.

The results of the test showed a EC50 48 h > 125 mg/l.

Based on the criteria of the Regulation 1272/2008, the substance does not required to be classfied for the environment.

Description of key information

There are two available studies performed both on Acid Yellow 23 and on another form of this dye.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
125 mg/L

Additional information

The key study has been performed for different concentration and based on the active ingredient a value of EC50 > 125 mg/l has been established.

Based on the criteria of the CLP Regulation (EC n. 1272/2008), the substance does not required the classification for environment.