Mixture of acrylic acid 4-[(chlorocarbonyl)oxy]butyl ester and 3-chloropropanoic acid 4-[(chlorocarbonyl)oxy]butyl ester

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and guideline study

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Reference 2

Reference Type:

other: re-evaluation

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

GLP compliance:

yes (incl. QA statement)

Remarks:

testiing lab.

Test type:

acute toxic class method

Test material

Test material information

Constituent 1

Reference substance name:

Acryloxibutylchlorformiat

IUPAC Name:

Acryloxibutylchlorformiat

Details on test material:

- Name of the test substance used in the study report: Acryloxibutylchlorformiat
- Purity: ca. 75%

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Young adult male and female rats with a comparable weight were used.
One animal per cage (type: stainless steel wire mesh cages, DK-III); animal identification using cage cards and group identification by tail marking.
They were housed in fully air-conditioned rooms with a room temperature of 20 - 24°C and a relative humidity of 30 - 70%. The day/night rhythm was 12 h light and 12 h dark.
The animals were offered a standardized animal laboratory diet as well as tap water ad libitum.
The animals were given no feed at least 16 hours before administration (water was available ad libitum).
Acclimatization period: at least one week

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

The test substance is sensitive to hydrolysis.
Form of administration: solution
Concentrations used: 4 and 40 g/100 ml
Administration volume: 5 ml/kg

Doses:

200, 2000 mg/kg

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

Observation period: 14 days
Individual body weights were recorded shortly before administration (day 0), weekly thereafter and at the end of the study (before fasting period).
Recording of signs and symptoms several times on the day of administration, at least once each working day.
A check for general observations and mortality was made twice each working day and once on weekends and on public holidays.
Necropsy at the last day of the observation period. Withdrawal of food at least 16 hours before killing with CO2; then necropsy of all animals that died before as soon as possible.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Remarks on result:

other: see attached document

Mortality:

Male animals: 200 mg/kg: no deaths; 2000 mg/kg: 1/3
Female animals: 200 mg/kg: no deaths; 2000 mg/kg: 1/3

Clinical signs:

other: Impaired and poor general state, dyspnea, apathy, staggering, twitching (females only), spastic gait, piloerection, smeared fur, diarrhea, smeared fur in the anogenital region (males only)

Gross pathology:

Animals that died: agonal congestion
Sacrificed animals: no pathologic findings noted