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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure is in accordance with national standard methods with acceptable restrictions (no GLP, no analytical measurement of the test substance)
Qualifier:
equivalent or similar to guideline
Guideline:
other: other guideline: rules of Dutch Standard Organization (NEN Nederlands Normalisatie Instituut 6506, 1980)
GLP compliance:
no
Analytical monitoring:
no
Details on sampling:
No data shown
Vehicle:
not specified
Details on test solutions:
No data shown
Test organisms (species):
Chlorella vulgaris
Details on test organisms:
The Log phase obtained from standardized laboratory cultures
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
No data shown
Test temperature:
The test temperature was 22 +/- 2 °C during the experiment.
pH:
No data shown
Dissolved oxygen:
No data shown
Salinity:
No data shown
Nominal and measured concentrations:
The test concentrations were nominal.
Details on test conditions:
Number of organism per group =ca. 5*10^4 cells/mL; continous ca. 13 W/m2 lighting
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
560 - 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
NAS-a
Basis for effect:
growth rate

The EC50 of the short-term toxicity test to Chlorella vulgaris were observed in the concentration intervals between 560 and 1000 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The toxicity test showed that NAS-a is not acutely tocic to Chlorella vulgaris. The 50% mortality (EC50) was observed in the concentartion intervals between 560 and 1000 mg/L indicating low toxicity to the selected species.
Executive summary:

The short-term toxicity of sodium aluminium silicate (NAS-a) to the fresh water aquatic algae Chlorella vulgaris was examined in accordance to the Dutch Standard Organization (NEN Nederlands Normalisatie Institut, 1980) guidelines. A 96 hours toxicity test was performed on a standardized laboratory culture containing ca. 5*10^4 cells/mL. The effect concentrations were nominal during the experiment. The EC50 value was determined according to Litchfield and Wilcoxon method. The tests were conducted under controlled temperature conditions at 22 ± 2°C with a continous light:dark cycle of ca. 13W/m2. Dosing was performed under static conditions. NAS-a showed low toxicity to the selected species with the EC50 being in the intervals between 560 and 1000 mg/L.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure is in accordance with national standard methods with acceptable restrictions (no GLP, no analytical measurement of the test substance)
Qualifier:
equivalent or similar to guideline
Guideline:
other: other guideline: rules of Dutch Standard Organization (NEN Nederlands Normalisatie Instituut 6506, 1980)
GLP compliance:
no
Analytical monitoring:
no
Details on sampling:
No data shown
Vehicle:
not specified
Details on test solutions:
No data shown
Test organisms (species):
Microcystis aeruginosa
Details on test organisms:
The Log phase obtained from standardized laboratory cultures
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
No data shown
Test temperature:
The test temperature was 23 +/- 2 °C during the experiment.
pH:
No data shown
Dissolved oxygen:
No data shown
Salinity:
No data shown
Nominal and measured concentrations:
The test concentrations were nominal.
Details on test conditions:
Number of organism per group =ca. 10^5 cells/mL; continous ca. 13 W/m2 lighting
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
180 - 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
NAS-a
Basis for effect:
growth rate

The EC50 of the short-term toxicity test to Chlorella vulgaris were observed in the concentration intervals between 180 and 320 mg/L.

Validity criteria fulfilled:
not applicable
Conclusions:
The toxicity test showed that NAS-a moderate toxicity to Microcystis aeruginosa. The 50% mortality (EC50) was observed in the concentartion intervals between 180 and 320 mg/L.
Executive summary:

The short-term toxicity of sodium aluminium silicate (NAS-a) to the fresh water aquatic algae Microcystis aeruginosa was examined in accordance to the Dutch Standard Organization (NEN Nederlands Normalisatie Institut, 1980) guidelines. A 96 hours toxicity test was performed on a standardized laboratory culture containing ca. 10^5 cells/mL. The effect concentrations were nominal during the experiment. The EC50 value was determined according to Litchfield and Wilcoxon method. The tests were conducted under controlled temperature conditions at 23 ± 2°C with a continous light:dark cycle of ca. 13W/m2. Dosing was performed under static conditions. NAS-a showed low toxicity to the selected species with the EC50 being in the intervals between 180 and 320 mg/L.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure is in accordance with national standard methods with acceptable restrictions (no GLP, no analytical measurement of the test substance)
Qualifier:
equivalent or similar to guideline
Guideline:
other: other guideline: rules of Dutch Standard Organization (NEN Nederlands Normalisatie Instituut 6506, 1980)
GLP compliance:
no
Analytical monitoring:
no
Details on sampling:
No data shown
Vehicle:
not specified
Details on test solutions:
No data shown
Test organisms (species):
other: Pseudomonas fluorescens
Details on test organisms:
The Log phase obtained from standardized laboratory cultures
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
8 h
Hardness:
No data shown
Test temperature:
The test temperature was 22 +/- 2 °C during the experiment.
pH:
No data shown
Dissolved oxygen:
No data shown
Salinity:
No data shown
Nominal and measured concentrations:
The test concentrations were nominal.
Details on test conditions:
Number of organism per group =ca. 10^8 cells/mL; lighting=none
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
3 200 - 5 600 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
NAS-a
Basis for effect:
other: Specific growth rate

The EC50 of the short-term toxicity test to Pseudomonas Fluorescens were observed in the concentration intervals between 3200 and 5600 mg/L.

Validity criteria fulfilled:
not applicable
Conclusions:
The toxicity test showed that NAS-a moderate toxicity to Pseudomonas Fluorescens. The 50% mortality (EC50) was observed in the concentartion intervals between 3200 and 5600 mg/L.
Executive summary:

The short-term toxicity of sodium aluminium silicate (NAS-a) to aquatic bacteria Pseudomonas Fluorescens was examined in accordance to the Dutch Standard Organization (NEN Nederlands Normalisatie Institut, 1980) guidelines. A 96 hours toxicity test was performed on a standardized laboratory culture containing ca. 10^8 cells/mL. The effect concentrations were nominal during the experiment and were determined according to Litchfield and Wilcoxon method. The tests were conducted under controlled temperature conditions at 22 ± 2°C with no light. Dosing was performed under static conditions. NAS-a showed low toxicity to the selected species with the EC50 being in the intervals between 3200 and 5600 mg/L.

Description of key information

The 96 hour EC50 of the read-across substance, NAS-a was in the intervals between 560 and 1000 mg/L for freshwater aquatic algae Chlorella Vulgaris and between 180 and 320 mg/L Microcystis aeruginosa. As intervals of toxicity are reported in this study, it is proposed to use the lower limit of EC50 and therefore the relatively more toxic value (560 mg/L Chlorella Vulgaris and 180 mg/L for Microcystis aeruginosa) for the purpose of classification. In addition, an 8 hour EC50 was determined to be between 3200 and 5600 mg/L for bacteria Pseudomonas fluorescens. NAS-a is not considered to be toxic to aquatic algae.

Key value for chemical safety assessment

EC50 for freshwater algae:
180 mg/L

Additional information

The acute toxicity of the read-across substance, NAS-a, to aquatic algae and bacteria was determined in accordance with rules of Dutch Standard Organization (NEN Nederlands Normalisatie Institut, 1980). The short-term toxicity test was carried out with three different species (2 aquatic algae and 1 bacteria): the algae Chlorella vulgaris and Microcystis aeruginosa and the bacteria Pseudomonas fluorescens. The effect concentrations were nominal during the study. The exposed algae were monitored over a 96 hours period to determine the concentration of the test substance which inhibit algal growth rate by 50% (EC50), while the EC 50 for the bacteria was determined after a 8 hours exposure period. The 96 hours EC50 was in the intervals of intervals between 560 and 1000 mg/L for freshwater aquatic algae Chlorella Vulgaris and between 180 and 320 mg/L Microcystis aeruginosa. The 8 hours EC50 was determined to be between 3200 and 5600 mg/L. The read-across substance is not considered to be toxic to aquatic algae and bacteria. Read-across between the registered substance and the test substance is considered justified owing the structural similarity of the two substances. The registered substance and NAS-a will release silicon, aluminium, and respectively magnesium and sodium. As NAS-a is more water soluble than Cordierite, its aquatic toxicity represents the worst case scenario.