Cordierite (Mg2[Al4O3(SiO3)5])

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: comparable to guideline study with acceptable restrictions (no analytical monitoring of the test concentrations, no purity of the test substance given)

Qualifier:

equivalent or similar to guideline

Guideline:

other: in analogy to rules of Dutch Standard Organization (NEN Nederlands Normalisatie Institut 6504, 1980)

Deviations:

not specified

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

Test solution were corrected for pH and oxygen content

Test organisms (species):

Poecilia reticulata

Details on test organisms:

The test organisms were 3 to 4 weeks old and were obtained from standardized laboratory cultures

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

Test Concentrations in mg/L Hardness after 72hr in mg CaCO3 and MgSO4/L
- 220
10 220
32 220
100 205
320 150

Test temperature:

The temperature was 23 +/- 2 °C during the experiment

pH:

No data shown

Dissolved oxygen:

No data shown

Salinity:

No data shown

Nominal and measured concentrations:

The tested concentrations were nominal

Details on test conditions:

Lighting: circadic
Renewing rate: 1x per day

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 800 - 3 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

NAS-a

Basis for effect:

mortality (fish)

Details on results:

No data shown

Results with reference substance (positive control):

No data shown

Reported statistics and error estimates:

No data shown

Sublethal observations / clinical signs:

The LC50 of the short-term toxicity test to Poecilia Reticultata were observed in the concentration intervals between 1800 -3200 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The toxicity test showed that NAS-a is not acutely tocic to Poecilia Reticulata. The 50% mortality (LC50) was observed in the concentartion intervals between 1800 and 3200 mg/L indicating low toxicity to the selected species.

Executive summary:

The short-term toxicity of sodium aluminium silicate (NAS-a) to the fresh water fish species Poecilia Reticulata was examined in accordance to the Dutch Standard Organization (NEN Nederlands Normalisatie Institut, 1980) guidelines which is similar to the OECD 203 guideline (Fish Acute Toxicity test). A 96 hours toxicity test was performed on 10 organisms per gorup from standardized laboratory cultures. The concentrations were not measured during the experiment. The LC50 value was determined according to Litchfield and Wilcoxon method. The tests were conducted under controlled temperature conditions at 23 ± 2°C with a circadiac light:dark cycle. Dosing was performed under semistatic conditions at the renewing rate of 1 per day. NAS-a showed low toxicity to the selected species with the LC50 being in the intervals between 1800 and 3200 mg/L.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: comparable to guideline study with acceptable restrictions (no analytical monitoring of the test concentrations, no purity of the test substance given)

Qualifier:

equivalent or similar to guideline

Guideline:

other: in analogy to rules of Dutch Standard Organization (NEN Nederlands Normalisatie Institut 6504, 1980)

GLP compliance:

no

Remarks:

Peer-reviewed publication

Analytical monitoring:

no

Details on sampling:

No data shown

Vehicle:

not specified

Details on test solutions:

Test solutions were corrected for oxygen content

Test organisms (species):

Oryzias latipes

Details on test organisms:

Test organisms were 4 to 5 weeks old and were obtained from standardized laboratory cultures

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No data shown

Hardness:

No data shown

Test temperature:

The tempearture was 23 +/- 2 °C during the experiment

pH:

No data shown

Dissolved oxygen:

No data shown

Nominal and measured concentrations:

The test concentrations were nominal.

Details on test conditions:

Lighting was circadiac with renewing rate of 1 per day under semi-static test conditions. Numeber of organisms per group was 10.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3 000 - 5 600 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

NAS-a

Basis for effect:

mortality (fish)

Details on results:

No data shown

Results with reference substance (positive control):

No data shown

Reported statistics and error estimates:

No data shown

Sublethal observations / clinical signs:

The LC50 of the short-term toxicity test to Oryzias latipes were observed in the concentration intervals between 3200 and 5600 mg/L.

Conclusions:

The test material NAS-a showed a relatively low acute toxicity to the selected test species Oryzias latipes with the LC50 ranging from 3,200 and 5,600 mg/L

Executive summary:

The LC50 derived from the short-term toxicity of sodium aluminium silicate (NAS-a) to the fresh water fish species Oryzias latipes showed a relatively low acute toxicity. The test was carried out in accordance to the Dutch Standard Organization (NEN Nederlands Normalisatie Institut, 1980) guidelines which is similar to the OECD 203 guideline (Fish Acute Toxicity test). A 96 hours toxicity test was performed on 10 organisms per gorup from standardized laboratory cultures. The concentrations were not measured during the experiment. The LC50 value was determined according to Litchfield and Wilcoxon method. The tests were conducted under controlled temperature conditions at 23 ± 2°C with a circadiac light:dark cycle. Dosing was performed under semistatic conditions at the renewing rate of 1 per day. NAS-a showed low toxicity to the selected species with the LC50 being in the intervals between 3,200 and 5,600 mg/L.

Description of key information

The 96 hour LC50 of the read-across substance, NAS-a (Zeolite 4A,  was in the intervals of 1800 and 3200mg/L for fresh water fish species Poecilia Reticulata and in the intervals of 3200 and 5600 mg/L for fresh water fish species Oryzias latipes. As ranges of toxicity were provided in the study it is proposed to use the most conservative LC50 (1800 mg/L for Poecilia Reticulata and 3200 mg/L for Oryzias latipes) for the purpose of the classification. However, even with the most conservative LC50, NAS-a, is not considered to be toxic to fish based on the finding of this study.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 800 mg/L

Additional information

The acute toxicity of NAS-a to fish was determined in accordance with rules of Dutch Standard Organization (NEN Nederlands Normalisatie Institut 6504, 1980) which is similar to the OECD 203 guideline (Fish Acute Toxicity test). The species used for the test were Poecilia Reticulata and Oryziaslatipes which were tested under semi-static conditions for 96 hours at the renewing rate of 1 per day. The tests were conducted under controlled temperature conditions at 23 ± 2°C with a circadiac light:dark cycle. NAS-a showed low toxicity to the selected species with the LC50 being in the intervals between 1800 and 3200 mg/L for Poecilia Reticulata and in the intervals of 3200 and 5600 mg/L for Oryzias latipes. As an interval of LC50 was provided, it is proposed to use the lowest toxicity value of LC50 (1800 mg/L for Poecilia Reticulata and 3200 mg/L for Oryzias latipes) as this is the most conservative approach. Read-across between the registered substance and the test substance is considered justified owing the structural similarity of the two substances. The registered substance and NAS-a will release silicon, aluminium, and respectively magnesium and sodium. As NAS-a is more water soluble than Cordierite, its aquatic toxicity represents the worst case scenario.