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EC number: 811-460-4 | CAS number: 53606-41-0
The test substance, 2-methoxy-5-nitrophenyl acetate, was tested for growth inhibition on algae Desmodesmus subspicatus. The test was performed according to method C.3. - Freshwater Algae and Cyanobacteria, Growth Inhibition Test, Commission Regulation (EC) No. 761/2009, Annex IV. The preliminary test was performed in range of test substance nominal concentrations 1 – 100 mg·L-1. The analytical results showed, that the test substance 2-methoxy-5-nitrophenyl acetate was not stable in the test medium at conditions of the test in concentration range 1 – 100 mg·L-1 . Based on the toxicity of the test substance found in preliminary test, the full test was performed in appropriate concentration range. The full test was performed in range of test substance nominal concentrations 5 – 62 mg·L-1. Samples for analytical determination were taken at the beginning, after 24 hours, 48 hours and at the end of the test. The analytical results showed that the test substance 2-methoxy-5-nitrophenyl acetate was not stable in test medium at conditions of the test. With respect to this observation the geometric mean concentrations were used for results presentation and evaluation. Test results: 72 hour – ErC50 = 1.53 mg·L-1 (geometric mean concentration) (95% confidence limit: 1.04 – 2.38 mg·L-1) 72 hour – EyC50 = 0.16 mg·L-1 (geometric mean concentration) (95% confidence limit: 0.02 – 0.38 mg·L-1) 72 hour – NOECr = 0.27 mg·L-1 (geometric mean concentration) 72 hour – NOECy < 0.18 mg.L-1
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