Phenol, 2-methoxy-5-nitro-, 1-acetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-methoxy-5-nitrophenyl acetate

Cas Number:

53606-41-0

Molecular formula:

C9H9NO5

IUPAC Name:

2-methoxy-5-nitrophenyl acetate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

20°C (max. deviation of 2°C) with variation of 1°C at aach individual test

pH:

7.7 (should be 7.8 with max. deviation of 0.2 for semistatic test)

Dissolved oxygen:

8.0 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

51.5 mg/L

Nominal / measured:

meas. (TWA)

Basis for effect:

mobility

Remarks on result:

other: 50.0-53.0 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

26.4 mg/L

Nominal / measured:

meas. (TWA)

Basis for effect:

mobility

Remarks on result:

other: 20.5-32.3 mg/L

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

7.3 mg/L

Nominal / measured:

meas. (TWA)

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

4.9 mg/L

Nominal / measured:

meas. (TWA)

Basis for effect:

mobility

Effect concentrations 5

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

73.9 mg/L

Nominal / measured:

meas. (TWA)

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

73.9 mg/L

Nominal / measured:

meas. (TWA)

Basis for effect:

mobility

Results with reference substance (positive control):

The results of the verification test with K2Cr2O7, are the following:
24 hour – EC50 = 1.95 mg·L-1(95% confidence limit: 1.75 – 2.15 mg·L-1)
48 hour – EC50 = 0.86 mg·L-1(95% confidence limit: 0.70 – 1.02 mg·L-1)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The test substance, 2-methoxy-5-nitrophenyl acetate, was tested in acute immobilisation test on Daphnia magna.

The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.

The preliminary test was performed in range of the test substance nominal concentrations 1 – 100 mg·L-1. The analytical results showed, that the test substance 2-methoxy-5-nitrophenyl acetate was not stable in dilution water in the conditions of the test. Based on toxicity and instability of the test substance found in preliminary test, the definitive test was performed in appropriate concentration range and as semistatic.

The semistatic definitive test was performed in range of the test substance nominal concentrations 9 - 100 mg·L-1. The test solutions were renewed after 24 hours. The test substance concentrations was measured at the beginning, after 24 hours (before and after renewal) and at the end of the test.

There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values.

Because concentrations of the test substance have not been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, time-weighted mean concentrations were used for all evaluations and results.