Registration Dossier
Registration Dossier
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EC number: 931-468-2 | CAS number: 1190625-94-5
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
3 h EL50 >1000 mg/L, NOEL 1000 mg/L; OECD 209, EU Method C.11 and ISO Standard 8192
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
In the key study, the potential for the test material to cause toxicity to activated sludge was investigated in a study conducted in accordance with the standardised guidelines OECD 209, EU Method C.11 and ISO Standard 8192 under GLP conditions. The study was awarded a reliability score of 1 in accordance with the principles for assessing data quality set forth by Klimisch et al. (1997).
The influence of the test material on the respiration rate of activated sludge of a predominantly domestic sewage was investigated in a combined limit/range-finding test after a contact time of 3 hours.
Loading rates of 10, 100 and 1000 mg/L were tested. The highest loading rate was tested in triplicate; lower loading rates consisted of one replicate. Furthermore, at 1000 mg/L an abiotic control (1 replicate) and three replicates with a nitrification inhibitor were tested. Responses were compared to the blank and nitrification controls.
The test material was not sufficiently soluble to allow the preparation of a 10 g/L stock solution in water. Therefore, test material and Milli-RO water mixtures were magnetically stirred for a period of approximately 24 hours. Subsequently, synthetic medium, sludge and Milli-RO water were added, resulting in the required loading rates. Optimal contact between the test material and test medium was ensured by applying continuous aeration and stirring during the 3 hour exposure period. Thereafter, oxygen consumption was recorded for 10 to 11 minutes.
No statistically significant inhibition of the respiration rate of the sludge was recorded at a loading rate of 1000 mg/L. The batch of activated sludge was tested for sensitivity with the reference material 3,5-dichlorophenol, and showed normal sensitivity.
The study met all the acceptability criteria was considered valid.
Under the conditions of this study, the test material was not toxic to waste water (activated sludge) bacteria at a loading rate of 1000 mg/L (NOEL). The EL50 was above 1000 mg/L.
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