2-chloro-1,1-dimethoxyethane

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline Study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethane, 2-Chloro-1, 1-Dimethoxy-

IUPAC Name:

Ethane, 2-Chloro-1, 1-Dimethoxy-

Constituent 2

Reference substance name:

97-97-02

IUPAC Name:

97-97-02

Constituent 3

Reference substance name:

2-chloro-1,1-dimethoxyethane

EC Number:

202-624-0

EC Name:

2-chloro-1,1-dimethoxyethane

Cas Number:

97-97-2

Molecular formula:

C4H9ClO2

IUPAC Name:

2-chloro-1,1-dimethoxyethane

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Chloroacetaldehyde dimethylacetal
- Physical state: colourless liquid
- Analytical purity: 98%

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
- Water solubility: 52.0 g/L at 20°C
- vapor pressure: 9 hPA at 20°C; 50 hPa at 50°C

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution of 0.5 g/L and 0.2 g/L
test concentration (max. 100mL) and 6.4mL synthetic sewage with tab water are filled up to a volume of 120mL in 200mL flasks. The end volume of 200mL is reached after adding 80mL inoculum.
- Controls: 3,5-Dichlorophenol (pH 7-8)

Test organisms

Test organisms (species):

activated sludge

Details on inoculum:

- Preparation of inoculum for exposure: set down of sludge to separate rough sludge parts. The rest of the sludge is filled up with tab water in a 2L PE-bottle. The procedure will be repeated 3 times.
- Pretreatment: aeration at 20 +/- 2 °C. pH proved and if necessary adjusted to 6-8

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

30 min

Test conditions

Hardness:

no data

Test temperature:

20 +/- 2°C

pH:

6-7

Dissolved oxygen:

oxygen depletion in mg O2/L * h:
stock 1: 38.0
stock 2: 64.5

Salinity:

no data

Nominal and measured concentrations:

12.5, 25, 37.5, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: flask
- Aeration: yes

TEST MEDIUM / WATER PARAMETERS
- synthetic sewage g/L: 16.0g Pepton; 11.0g meat extract; 3.0g urea; 0.7g NaCl; 0.4g CaCl2 - 2 H2O; 0.2g MgSO4 - 7 H2O; 2.8g K2HPO4

Reference substance (positive control):

yes

Remarks:

3.5 Dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

22.9 other: mg O2/L

Nominal / measured:

meas. (initial)

Conc. based on:

other: reference substance

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Details on results:

Chloroacetaldehyde dimethylacetal was tested in an activated sludge respiration inhibition test according to OECD guideline 209. At the used concentrations no inhibition of the oxygen depletion microorganisms was shown. Consequently no EC20-, EC50- and EC80 value can be recorded.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Chloroacetaldehyde dimethylacetal was tested in an activated sludge respiration inhibition test according to OECD guideline 209. At the used concentrations no inhibition of the oxygen depletion microorganisms was shown. Consequently no EC20-, EC50- and EC80 value can be recorded.

Executive summary:

Chloroacetaldehyde dimethylacetal was tested in an activated sludge respiration inhibition test according to OECD guideline 209. At the used concentrations no inhibition of the oxygen depletion microorganisms was shown. Consequently no EC20-, EC50- and EC80 value can be recorded.