2-chloro-1,1-dimethoxyethane

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline Study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethane, 2-Chloro-1,1-Dimethoxy-

IUPAC Name:

Ethane, 2-Chloro-1,1-Dimethoxy-

Constituent 2

Reference substance name:

97-97-02

IUPAC Name:

97-97-02

Constituent 3

Reference substance name:

2-chloro-1,1-dimethoxyethane

EC Number:

202-624-0

EC Name:

2-chloro-1,1-dimethoxyethane

Cas Number:

97-97-2

Molecular formula:

C4H9ClO2

IUPAC Name:

2-chloro-1,1-dimethoxyethane

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Chloroacetaldehyde dimethylacetal
- Molecular formula: C4H9ClO2
- Molecular weight: 124.57 g/mol
- Physical state: clear liquid
- Storage condition of test material: chilled dark place

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: <-73°C
- Water solubility: 49g/L
- Solubility in organic solvents: Hexane >100g/L (by vision); Chloroform >100 g/L (by vision); Ethyl acetate >100 g/L (by vision); Methanol >100 g/L (by vision)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: City Sewage (Returned sludge of sewage plants were collected), Rivers, lake and sea (Surface water and surface soil which is in contact with atmosphere were collected)
- Method of cultivation: ~30min after ceasing the aeration to the sludge mixture, supernatant corresponding to about 1/3 of the whole volume was removed. Then, equal volume of dechlorination water was added to the remaining portion and aerated again, followed by addition of synthetic sewage. Concentration of that portion was to be 0.1 (W/V)%. Daily repetition of this procedure and carried out at 25 +/- 2 °C.
- Preparation of inoculum for exposure: 5L of the filtrate of the supernatant of an activated sludge in the present use was mixed with 500mL of the filtrate of the supernatant of a newly collected sludge respectively and the mixture was cultured at pH 7.0 +/- 1.0 under the sufficient aeration (with prefiltered open air).
- Concentration of sludge:30 mg/L
- Water filtered: yes/no
- Type and size of filter used, if any:

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Details on study design:

TEST CONDITIONS
- Test temperature: 25 +/- 1 °C
- pH: 7 +/- 1
- Suspended solids concentration: 3700 mg/L
- Continuous darkness: yes/no

TEST SYSTEM
- Instrument for cultivation: closed system oxygen consumption measuring apparatus (Coulometer: Ohkura Electronic Co., Ltd.)
- Vessel: 300 mL in volume
- Absorbent for carbon dioside: Soda lime No. 1 (extra pure reagent, Wako Pure Chemical Industries, Ltd.)
- Stirring method: test solution was stirred by a magnetic stirrer

CONTROL AND BLANK SYSTEM
- Abiotic sterile control: Microflora in the activated sludge was microscopically observed and sludge with no abnormal symptom was used for the test.
- Control blank: A Test vessel containing 300 mL of basal culture medium into which was not added test substance nor aniline.

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (TOC removal)

Value:

6

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

The test substance was not biodegradable under the present test conditions.

Executive summary:

The test article, Chloroacetaldehyde dimethylacetal, was tested in the Biodegradability by microorganisms according to OECD Guideline Study 301 C.

 

Biodegradability test:

Test conditions:

-      concentration of test substance: 100 mg/L

-      concentration of activated sludge (as the concentration of suspended solid): 30 mg/L

-      volume of test solution: 300 mL

-      cultivating temperature: 25 +/- 1°C

-      cultivation duration: 28 days

 

Measurement and analysis:

-      Measurement of biochemical oxygen demand (BOD) by closed system oxygen consumption measuring apparatus

-      Determination of total organic carbon by total organic carbon analyzer (TOC)

-      Determination of test substance by gas chromatography (GC)

 

Test results:

-      Percentage biodegradation by BOD: 6%, 5%, 6% -> average 6%

-      Percentage biodegradation by TOC: 0%, 1%, 0% -> average: 0%

-      Percentage biodegradation by GC: 1%, 3%, 4% -> average: 3%

 

Stability of the test substance:

It was confirmed that the test substance was stable under the storage condition.