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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study meets scientific standards with acceptable restrictions (limited documentation, exposure for 1-15 min and 20h)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Principles of method if other than guideline:
Skin irritation was tested using an internal standard method (BASF test). White Vienna rabbits were used. Usually, 2 animals were treated for 1, 5 and 15 minutes and 2 other animals for 20 hours using occlusive conditions. An application site of 2.5 X 2.5 cm was covered with the liquid test substance.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-dimethylaminopropiononitrile
EC Number:
217-090-4
EC Name:
3-dimethylaminopropiononitrile
Cas Number:
1738-25-6
Molecular formula:
C5H10N2
IUPAC Name:
3-(dimethylamino)propanenitrile
Details on test material:
- Name of test material (as cited in study report): N,N-Dimethyl-amino-propionitril
- Physical state: liquid

Test animals

Species:
rabbit
Strain:
Vienna White

Test system

Type of coverage:
occlusive
Preparation of test site:
shaved
Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
ca. 0.5 mL
Duration of treatment / exposure:
Experiment 1: 1, 5, or 15 minutes
Experiment 2: 20 h
Observation period:
Experiment 1: up to 8 days
Experiment 2: up to 8 days
Number of animals:
2 per experiment
Details on study design:
TEST SITE
- Area of exposure: An application site of 2.5 X 2.5 cm was covered with the liquid (37°C; approx. 1 ml)

REMOVAL OF TEST SUBSTANCE
- Washing:
Experiment 1: washing was done in the 1 min, 5 min and 15 min test directly after exposure. Lutrol 50% (mild detergent) was used.
Experiment 2: No washing was done after the 20 h treatment.

SCORING SYSTEM: Descriptive scores of the raw data have been converted to Draize numerical scores

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Remarks:
20 h exposure
Time point:
other: 24 - 48 - 72 h
Score:
0.5
Max. score:
4
Reversibility:
fully reversible within: 4 d
Remarks on result:
other: 1/2 animals without any signs of irritation
Irritation parameter:
edema score
Basis:
mean
Remarks:
20 h exposure
Time point:
other: 24 - 48 - 72 h
Score:
0
Max. score:
4
Reversibility:
other: no effects

Any other information on results incl. tables

Exposure to one, five or 15 min caused no erythema or edema.

Applicant's summary and conclusion