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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex
EC Number:
413-650-9
EC Name:
Phthalocyanine-N-[3-(diethylamino)propyl]sulfonamide copper complex
Cas Number:
93971-95-0
Molecular formula:
Can not be identified
IUPAC Name:
hexacopper(2+) 5-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,14-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6,15-bis({[3-(diethylamino)propyl]sulfamoyl})-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 6-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 7-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide 8-{[3-(diethylamino)propyl]sulfamoyl}-2,11,20,29,37,38,39,40-octaazanonacyclo[28.6.1.1^{3,10}.1^{12,19}.1^{21,28}.0^{4,9}.0^{13,18}.0^{22,27}.0^{31,36}]tetraconta-1,3(40),4,6,8,10,12,14,16,18,20,22,24,26,28(38),29,31,33,35-nonadecaene-37,39-diide
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
Direct dispersion in water with the aid of shielded propeller-stirres. 2.0 g of test material were dispersed in 20 litters of water with the aid of shielded propeller-stirreres to give a 100 mg/l test concentration. 1% Tween 80-dimethylsulphoxide at a concentration of 100 μl/l was added to aid dispersal of the test material.

Test organisms

Test organisms (species):
other: RAINBOW TROUT
Details on test organisms:
-Souce : Parkwood Trout Farm, Wigmore, Kent, U.K.
-Mean standard length : 5.3 cm (SD = 0.7 cm)
-Mean weight : 1.57g (SD = 0.45 g)

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
96 h

Test conditions

Hardness:
approximately 50 mg/L as CaCO3
Test temperature:
14 °C
pH:
7.5 - 7.6
Dissolved oxygen:
10.0 -10.1 mg O2/L
Nominal and measured concentrations:
Nominal concentration : 100 mg/L
Concentration found 0 hour : 94.3 mg/L, 96.7 mg/L
Concentration found 24 hours : 103 mg/L, 94.4 mg/L
Concentration found 96 hours : 89.6 mg/L, 92.1 mg/L
Details on test conditions:
Test water : Laboratory tap water, dechlorinated by the addition of sodium thiosulphate.
Test vessels : Glass aquaria holding 20 l of test media.
Experimental design : 1 test concentration in duplicate plus 1 control and 1 solvent control (including 100 μl/l of auxiliary solvent). 20 animals per test concentration.
Method of initiation : Fish were placed, at random, in prepared test media.
Loading : 0.79 g bodyweight/litre.
Photoperiod : 16h light, 8h dark.
Aeration : By shielded propeller-stirrers.
Test concentration : 100 mg/l, in duplicate.
Media renewal : Daily.
Criteria of death : Absence of (i) respiratory movement and (ii) response to a physical stimulation.

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

  Cumulative Mortality Date for Rainbow Trout Expose for 96 hours.
 Concentration  Cumulative mortality (initial population : 10)               
 mg/l  3h  6h  24h  48h  72h  96h
 Control  0  0  0  0  0  0
 Solvent control  0  0  0  0  0  0
 100 R1  0  0  0  0  0  0
 100 R2  0  0  0  0  0  0
             R1, R2 = Replicates R1 and R2      

Applicant's summary and conclusion

Conclusions:
LC50 > 100mg/L
No Observed Effect Concentration(NOEC) ≧100mg/L